PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS

A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purif...

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Detalles Bibliográficos
Autores principales: Henn, Fritz A., Hansson, Hans-Arne, Hamberger, Anders
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108764/
https://www.ncbi.nlm.nih.gov/pubmed/4402165
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author Henn, Fritz A.
Hansson, Hans-Arne
Hamberger, Anders
author_facet Henn, Fritz A.
Hansson, Hans-Arne
Hamberger, Anders
author_sort Henn, Fritz A.
collection PubMed
description A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic analyses showed a 4–5-fold enrichment in plasma membrane markers, and a 10–15% contamination of mitochondrial and microsomal material. Electron micrographs of the membrane fraction confirmed the enzymatic data Fragmented membranes were found, mainly in vesicular form No ribosomes, but a few mitochondria and some multilamellar membranes were seen
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spelling pubmed-21087642008-05-01 PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS Henn, Fritz A. Hansson, Hans-Arne Hamberger, Anders J Cell Biol Article A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic analyses showed a 4–5-fold enrichment in plasma membrane markers, and a 10–15% contamination of mitochondrial and microsomal material. Electron micrographs of the membrane fraction confirmed the enzymatic data Fragmented membranes were found, mainly in vesicular form No ribosomes, but a few mitochondria and some multilamellar membranes were seen The Rockefeller University Press 1972-06-01 /pmc/articles/PMC2108764/ /pubmed/4402165 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Henn, Fritz A.
Hansson, Hans-Arne
Hamberger, Anders
PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS
title PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS
title_full PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS
title_fullStr PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS
title_full_unstemmed PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS
title_short PREPARATION OF PLASMA MEMBRANE FROM ISOLATED NEURONS
title_sort preparation of plasma membrane from isolated neurons
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108764/
https://www.ncbi.nlm.nih.gov/pubmed/4402165
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AT hanssonhansarne preparationofplasmamembranefromisolatedneurons
AT hambergeranders preparationofplasmamembranefromisolatedneurons

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