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LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE
Low temperature ultramicroincineration was employed to determine the morphological localization of "structure-bound" mineral and/or metallic elements within biological cells at the electron microscope level. This technique chemically removes organic material from thin sections of tissues w...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1972
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108797/ https://www.ncbi.nlm.nih.gov/pubmed/4116522 |
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author | Hohman, Wayne Schraer, Harald |
author_facet | Hohman, Wayne Schraer, Harald |
author_sort | Hohman, Wayne |
collection | PubMed |
description | Low temperature ultramicroincineration was employed to determine the morphological localization of "structure-bound" mineral and/or metallic elements within biological cells at the electron microscope level. This technique chemically removes organic material from thin sections of tissues with reactive, excited oxygen instead of heat as used in a furnace. The remaining ash representing the mineral/metallic ultrastructure of cells is advantageous for ultrastructural studies because incineration without applying heat is less destructive than the burning associated with high temperatures. This low temperature incineration method was applied to thin-sectioned avian shell gland mucosa, a calcium transporting system, as a sample tissue. The results include: recognition of many subcellular organelles in the ash patterns, identification of dense, ash-containing granules (possibly organic-metallic complexes) in epithelial cells which may be involved in calcium transport, description of ashed erythrocytes and collagen, comparison of ashed glutaraldehyde fixed tissue with and without osmium postfixation, description of lead-stained cells after ashing, demonstration that ash preservation is dependent upon section thickness, illustration of the fine resolution obtainable because the ash residues remain relatively near their in situ origins, discussion of technical problems in this relatively new field, and demonstration of the presence of Ca and P in the ash with electron microprobe X-ray analysis. |
format | Text |
id | pubmed-2108797 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1972 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21087972008-05-01 LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE Hohman, Wayne Schraer, Harald J Cell Biol Article Low temperature ultramicroincineration was employed to determine the morphological localization of "structure-bound" mineral and/or metallic elements within biological cells at the electron microscope level. This technique chemically removes organic material from thin sections of tissues with reactive, excited oxygen instead of heat as used in a furnace. The remaining ash representing the mineral/metallic ultrastructure of cells is advantageous for ultrastructural studies because incineration without applying heat is less destructive than the burning associated with high temperatures. This low temperature incineration method was applied to thin-sectioned avian shell gland mucosa, a calcium transporting system, as a sample tissue. The results include: recognition of many subcellular organelles in the ash patterns, identification of dense, ash-containing granules (possibly organic-metallic complexes) in epithelial cells which may be involved in calcium transport, description of ashed erythrocytes and collagen, comparison of ashed glutaraldehyde fixed tissue with and without osmium postfixation, description of lead-stained cells after ashing, demonstration that ash preservation is dependent upon section thickness, illustration of the fine resolution obtainable because the ash residues remain relatively near their in situ origins, discussion of technical problems in this relatively new field, and demonstration of the presence of Ca and P in the ash with electron microprobe X-ray analysis. The Rockefeller University Press 1972-11-01 /pmc/articles/PMC2108797/ /pubmed/4116522 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Hohman, Wayne Schraer, Harald LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE |
title | LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE |
title_full | LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE |
title_fullStr | LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE |
title_full_unstemmed | LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE |
title_short | LOW TEMPERATURE ULTRAMICROINCINERATION OF THIN-SECTIONED TISSUE |
title_sort | low temperature ultramicroincineration of thin-sectioned tissue |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108797/ https://www.ncbi.nlm.nih.gov/pubmed/4116522 |
work_keys_str_mv | AT hohmanwayne lowtemperatureultramicroincinerationofthinsectionedtissue AT schraerharald lowtemperatureultramicroincinerationofthinsectionedtissue |