CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells

Measurements of actinomycin-(3)H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G(2) and mitosis, and then increases gradually throughout the subsequ...

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Detalles Bibliográficos
Autores principales: Pederson, Thoru, Robbins, Elliott
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108799/
https://www.ncbi.nlm.nih.gov/pubmed/5076779
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author Pederson, Thoru
Robbins, Elliott
author_facet Pederson, Thoru
Robbins, Elliott
author_sort Pederson, Thoru
collection PubMed
description Measurements of actinomycin-(3)H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G(2) and mitosis, and then increases gradually throughout the subsequent G(1) interval. Since this pattern was evident in experiments with living cells, ethanol-fixed cells, and isolated nuclei, but not with purified DNA, the actinomycin binding profile may reflect changes in the degree of association between DNA and chromosomal proteins at different stages of the cell cycle.
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spelling pubmed-21087992008-05-01 CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells Pederson, Thoru Robbins, Elliott J Cell Biol Article Measurements of actinomycin-(3)H binding in synchronized HeLa cells reveal that the binding capacity of chromatin decreases progressively during the S phase despite a doubling of nuclear DNA content, reaches a minimal level during G(2) and mitosis, and then increases gradually throughout the subsequent G(1) interval. Since this pattern was evident in experiments with living cells, ethanol-fixed cells, and isolated nuclei, but not with purified DNA, the actinomycin binding profile may reflect changes in the degree of association between DNA and chromosomal proteins at different stages of the cell cycle. The Rockefeller University Press 1972-11-01 /pmc/articles/PMC2108799/ /pubmed/5076779 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Pederson, Thoru
Robbins, Elliott
CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells
title CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells
title_full CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells
title_fullStr CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells
title_full_unstemmed CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells
title_short CHROMATIN STRUCTURE AND THE CELL DIVISION CYCLE : Actinomycin Binding in Synchronized HeLa Cells
title_sort chromatin structure and the cell division cycle : actinomycin binding in synchronized hela cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108799/
https://www.ncbi.nlm.nih.gov/pubmed/5076779
work_keys_str_mv AT pedersonthoru chromatinstructureandthecelldivisioncycleactinomycinbindinginsynchronizedhelacells
AT robbinselliott chromatinstructureandthecelldivisioncycleactinomycinbindinginsynchronizedhelacells

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