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BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm

In thermal denaturation experiments on sperm cells, described in the accompanying paper, it was found that squid sperm, when melted, lose both birefringence and morphological shape. Bull sperm, on the other hand, show no change of morphology, but their initial negative birefringence becomes positive...

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Detalles Bibliográficos
Autores principales: Bendet, Irwin J., Bearden, James
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108801/
https://www.ncbi.nlm.nih.gov/pubmed/5085774
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author Bendet, Irwin J.
Bearden, James
author_facet Bendet, Irwin J.
Bearden, James
author_sort Bendet, Irwin J.
collection PubMed
description In thermal denaturation experiments on sperm cells, described in the accompanying paper, it was found that squid sperm, when melted, lose both birefringence and morphological shape. Bull sperm, on the other hand, show no change of morphology, but their initial negative birefringence becomes positive. Since this suggested the existence of form birefringence, the influence of solvents of different refractive indices on the observed birefringence was investigated, using a new derivation of the Wiener form birefringence equations which allows direct comparison of Wiener's theory with experimental results. Bull sperm showed form birefringence both before and after melting, while squid sperm showed none. Quantitative application of the general form of the Wiener equations to these results gave values for the refractive index and intrinsic birefringence of bull sperm cells. Application of the specific forms of the Wiener equations showed that neither of these descriptions of idealized systems was adequate to describe completely the form birefringence of bull sperm, but that the equation for platelike submicroscopic structures was more nearly an accurate fit to the data than that for rodlike structures.
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spelling pubmed-21088012008-05-01 BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm Bendet, Irwin J. Bearden, James J Cell Biol Article In thermal denaturation experiments on sperm cells, described in the accompanying paper, it was found that squid sperm, when melted, lose both birefringence and morphological shape. Bull sperm, on the other hand, show no change of morphology, but their initial negative birefringence becomes positive. Since this suggested the existence of form birefringence, the influence of solvents of different refractive indices on the observed birefringence was investigated, using a new derivation of the Wiener form birefringence equations which allows direct comparison of Wiener's theory with experimental results. Bull sperm showed form birefringence both before and after melting, while squid sperm showed none. Quantitative application of the general form of the Wiener equations to these results gave values for the refractive index and intrinsic birefringence of bull sperm cells. Application of the specific forms of the Wiener equations showed that neither of these descriptions of idealized systems was adequate to describe completely the form birefringence of bull sperm, but that the equation for platelike submicroscopic structures was more nearly an accurate fit to the data than that for rodlike structures. The Rockefeller University Press 1972-11-01 /pmc/articles/PMC2108801/ /pubmed/5085774 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Bendet, Irwin J.
Bearden, James
BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm
title BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm
title_full BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm
title_fullStr BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm
title_full_unstemmed BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm
title_short BIREFRINGENCE OF SPERMATOZOA : II. Form Birefringence of Bull Sperm
title_sort birefringence of spermatozoa : ii. form birefringence of bull sperm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108801/
https://www.ncbi.nlm.nih.gov/pubmed/5085774
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