AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES

A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is s...

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Detalles Bibliográficos
Autores principales: Adelman, M. R., Blobel, Gunter, Sabatini, David D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108827/
https://www.ncbi.nlm.nih.gov/pubmed/4345164
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author Adelman, M. R.
Blobel, Gunter
Sabatini, David D.
author_facet Adelman, M. R.
Blobel, Gunter
Sabatini, David D.
author_sort Adelman, M. R.
collection PubMed
description A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction.
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spelling pubmed-21088272008-05-01 AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES Adelman, M. R. Blobel, Gunter Sabatini, David D. J Cell Biol Article A cell fractionation procedure is described which allows the preparation from rat liver of a rough microsome population containing almost 50% of the membrane-bound ribosomes of the tissue. The fraction is not contaminated with free ribosomes or smooth microsomes, and, by various other criteria, is suitable for studies of ribosome-membrane interaction. The Rockefeller University Press 1973-01-01 /pmc/articles/PMC2108827/ /pubmed/4345164 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Adelman, M. R.
Blobel, Gunter
Sabatini, David D.
AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES
title AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES
title_full AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES
title_fullStr AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES
title_full_unstemmed AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES
title_short AN IMPROVED CELL FRACTIONATION PROCEDURE FOR THE PREPARATION OF RAT LIVER MEMBRANE-BOUND RIBOSOMES
title_sort improved cell fractionation procedure for the preparation of rat liver membrane-bound ribosomes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108827/
https://www.ncbi.nlm.nih.gov/pubmed/4345164
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