THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings

Two different membrane fractions were obtained from a brush-border fraction of rat kidney cortex by using their different electrical surface charges in preparative free-flow electrophoresis. One membrane fraction contained only morphologically intact microvilli and was characterized by a high specif...

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Autores principales: Heidrich, Hans-G, Kinne, Rolf, Kinne-Saffran, Eva, Hannig, Kurt
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108866/
https://www.ncbi.nlm.nih.gov/pubmed/4261147
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author Heidrich, Hans-G
Kinne, Rolf
Kinne-Saffran, Eva
Hannig, Kurt
author_facet Heidrich, Hans-G
Kinne, Rolf
Kinne-Saffran, Eva
Hannig, Kurt
author_sort Heidrich, Hans-G
collection PubMed
description Two different membrane fractions were obtained from a brush-border fraction of rat kidney cortex by using their different electrical surface charges in preparative free-flow electrophoresis. One membrane fraction contained only morphologically intact microvilli and was characterized by a high specific activity of alkaline phosphatase. The other fraction morphologically resembled classical plasma membranes by possessing junctional complexes and a high Na-K-ATPase activity The contamination of the isolated membrane fractions by other cell organelles was extremely low These two fractions represent the apical (luminal) and the basal (interstitial) area of the renal proximal tubule cell membrane and clearly demonstrate the polarity of this cell.
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spelling pubmed-21088662008-05-01 THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings Heidrich, Hans-G Kinne, Rolf Kinne-Saffran, Eva Hannig, Kurt J Cell Biol Article Two different membrane fractions were obtained from a brush-border fraction of rat kidney cortex by using their different electrical surface charges in preparative free-flow electrophoresis. One membrane fraction contained only morphologically intact microvilli and was characterized by a high specific activity of alkaline phosphatase. The other fraction morphologically resembled classical plasma membranes by possessing junctional complexes and a high Na-K-ATPase activity The contamination of the isolated membrane fractions by other cell organelles was extremely low These two fractions represent the apical (luminal) and the basal (interstitial) area of the renal proximal tubule cell membrane and clearly demonstrate the polarity of this cell. The Rockefeller University Press 1972-08-01 /pmc/articles/PMC2108866/ /pubmed/4261147 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Heidrich, Hans-G
Kinne, Rolf
Kinne-Saffran, Eva
Hannig, Kurt
THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings
title THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings
title_full THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings
title_fullStr THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings
title_full_unstemmed THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings
title_short THE POLARITY OF THE PROXIMAL TUBULE CELL IN RAT KIDNEY : Different Surface Charges for the Brush-Border Microvilli and Plasma Membranes from the Basal Infoldings
title_sort polarity of the proximal tubule cell in rat kidney : different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108866/
https://www.ncbi.nlm.nih.gov/pubmed/4261147
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