INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM

The accumulation of sulfate-(35)S by Porphyridium aerugineum cells and subsequent appearance of solubilized capsular polysaccharide-(35)S in the growth medium were examined The uptake of label by the cells was largely light dependent. Pulse-chase experiments using log phase cells revealed a rapid la...

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Autores principales: Ramus, J., Groves, S. T.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1972
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108874/
https://www.ncbi.nlm.nih.gov/pubmed/5040864
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author Ramus, J.
Groves, S. T.
author_facet Ramus, J.
Groves, S. T.
author_sort Ramus, J.
collection PubMed
description The accumulation of sulfate-(35)S by Porphyridium aerugineum cells and subsequent appearance of solubilized capsular polysaccharide-(35)S in the growth medium were examined The uptake of label by the cells was largely light dependent. Pulse-chase experiments using log phase cells revealed a rapid labeling of solubilized capsular polysaccharide, recovered from the medium as the cetylpyridinium chloride precipitate Polyacrylamide gel electrophoresis of the polysaccharide-(35)S showed the sulfate to be firmly bound to an immobile fraction. Sephadex chromatography revealed the molecular weight of the polysaccharide to be in excess of 2 x 10(5). Acid hydrolysis of the polysaccharide-(35)S released sulfate-(35)S ion as evidenced by radioautography of thin layer chromatographs Preliminary electron microscope evidence suggests that the synthesis, movement, and deposition of the capsular polysaccharide on the cell surface are Golgi complex-mediated processes
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spelling pubmed-21088742008-05-01 INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM Ramus, J. Groves, S. T. J Cell Biol Article The accumulation of sulfate-(35)S by Porphyridium aerugineum cells and subsequent appearance of solubilized capsular polysaccharide-(35)S in the growth medium were examined The uptake of label by the cells was largely light dependent. Pulse-chase experiments using log phase cells revealed a rapid labeling of solubilized capsular polysaccharide, recovered from the medium as the cetylpyridinium chloride precipitate Polyacrylamide gel electrophoresis of the polysaccharide-(35)S showed the sulfate to be firmly bound to an immobile fraction. Sephadex chromatography revealed the molecular weight of the polysaccharide to be in excess of 2 x 10(5). Acid hydrolysis of the polysaccharide-(35)S released sulfate-(35)S ion as evidenced by radioautography of thin layer chromatographs Preliminary electron microscope evidence suggests that the synthesis, movement, and deposition of the capsular polysaccharide on the cell surface are Golgi complex-mediated processes The Rockefeller University Press 1972-08-01 /pmc/articles/PMC2108874/ /pubmed/5040864 Text en Copyright © 1972 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ramus, J.
Groves, S. T.
INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM
title INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM
title_full INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM
title_fullStr INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM
title_full_unstemmed INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM
title_short INCORPORATION OF SULFATE INTO THE CAPSULAR POLYSACCHARIDE OF THE RED ALGA PORPHYRIDIUM
title_sort incorporation of sulfate into the capsular polysaccharide of the red alga porphyridium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108874/
https://www.ncbi.nlm.nih.gov/pubmed/5040864
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