SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation

The synthesis of a surface protein has been studied in Paramecium through double-labeling experiments using [(14)C]- and [(3)H]leucine-labeled bacteria as the source of radioactive amino acid. Over a 4–5 h incubation period, the turnover rate was found to be higher than that of overall cell protein....

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Detalles Bibliográficos
Autores principales: Finger, Irving, Lavanchy, Patricia, Meany, Ann
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108887/
https://www.ncbi.nlm.nih.gov/pubmed/4682903
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author Finger, Irving
Lavanchy, Patricia
Meany, Ann
author_facet Finger, Irving
Lavanchy, Patricia
Meany, Ann
author_sort Finger, Irving
collection PubMed
description The synthesis of a surface protein has been studied in Paramecium through double-labeling experiments using [(14)C]- and [(3)H]leucine-labeled bacteria as the source of radioactive amino acid. Over a 4–5 h incubation period, the turnover rate was found to be higher than that of overall cell protein. In addition, the initial label is apparently utilized during the chase period, being incorporated into protein via a puromycin insensitive pathway.
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spelling pubmed-21088872008-05-01 SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation Finger, Irving Lavanchy, Patricia Meany, Ann J Cell Biol Article The synthesis of a surface protein has been studied in Paramecium through double-labeling experiments using [(14)C]- and [(3)H]leucine-labeled bacteria as the source of radioactive amino acid. Over a 4–5 h incubation period, the turnover rate was found to be higher than that of overall cell protein. In addition, the initial label is apparently utilized during the chase period, being incorporated into protein via a puromycin insensitive pathway. The Rockefeller University Press 1973-02-01 /pmc/articles/PMC2108887/ /pubmed/4682903 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Finger, Irving
Lavanchy, Patricia
Meany, Ann
SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation
title SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation
title_full SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation
title_fullStr SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation
title_full_unstemmed SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation
title_short SYNTHESIS OF PARAMECIUM SURFACE PROTEINS : I. Puromycin Insensitive Amino Acid Incorporation
title_sort synthesis of paramecium surface proteins : i. puromycin insensitive amino acid incorporation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108887/
https://www.ncbi.nlm.nih.gov/pubmed/4682903
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