A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure

The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37°C in Ringer's solutions containing from 0.1 to 100.0 µg/ml...

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Detalles Bibliográficos
Autores principales: Quinton, P. M., Philpott, C. W.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108935/
https://www.ncbi.nlm.nih.gov/pubmed/4687916
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author Quinton, P. M.
Philpott, C. W.
author_facet Quinton, P. M.
Philpott, C. W.
author_sort Quinton, P. M.
collection PubMed
description The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37°C in Ringer's solutions containing from 0.1 to 100.0 µg/ml of cationic polymers, and subsequently was fixed with 1% OsO(4) and examined with the electron microscope. All cationic polymers, at appropriate concentrations, produced similar changes in membrane structure. Adjacent membranes frequently were fused. Membrane structures such as microvilli lost rigidity. Cell membranes showed an apparent increase in permeability as judged by osmotically traumatized cells. These results indicate that fixed anionic sites play significant roles in stabilizing epithelial membrane structures.
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spelling pubmed-21089352008-05-01 A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure Quinton, P. M. Philpott, C. W. J Cell Biol Article The effects of several cationic polymers (poly-L-lysines, protamine, and histone) on rabbit gall bladder epithelial cells were studied to explore possible roles for negative sites in the membrane. The tissue was bathed for 30 min at 37°C in Ringer's solutions containing from 0.1 to 100.0 µg/ml of cationic polymers, and subsequently was fixed with 1% OsO(4) and examined with the electron microscope. All cationic polymers, at appropriate concentrations, produced similar changes in membrane structure. Adjacent membranes frequently were fused. Membrane structures such as microvilli lost rigidity. Cell membranes showed an apparent increase in permeability as judged by osmotically traumatized cells. These results indicate that fixed anionic sites play significant roles in stabilizing epithelial membrane structures. The Rockefeller University Press 1973-03-01 /pmc/articles/PMC2108935/ /pubmed/4687916 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Quinton, P. M.
Philpott, C. W.
A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure
title A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure
title_full A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure
title_fullStr A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure
title_full_unstemmed A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure
title_short A ROLE FOR ANIONIC SITES IN EPITHELIAL ARCHITECTURE : Effects of Cationic Polymers on Cell Membrane Structure
title_sort role for anionic sites in epithelial architecture : effects of cationic polymers on cell membrane structure
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108935/
https://www.ncbi.nlm.nih.gov/pubmed/4687916
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