[(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS

DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [(6)N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different...

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Detalles Bibliográficos
Autores principales: Gorovsky, Martin A., Hattman, Stanley, Pleger, Gloria Lorick
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108936/
https://www.ncbi.nlm.nih.gov/pubmed/4631666
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author Gorovsky, Martin A.
Hattman, Stanley
Pleger, Gloria Lorick
author_facet Gorovsky, Martin A.
Hattman, Stanley
Pleger, Gloria Lorick
author_sort Gorovsky, Martin A.
collection PubMed
description DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [(6)N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different strains of Tetrahymena, with approximately 0.65–0.80% of the adenine bases being methylated. The MeAde content of macronuclear DNA did not seem to vary in different physiological states. The level of MeAde in DNA isolated from micronuclei, on the other hand, was quite low (at least tenfold lower than in macronuclear DNA).
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spelling pubmed-21089362008-05-01 [(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS Gorovsky, Martin A. Hattman, Stanley Pleger, Gloria Lorick J Cell Biol Article DNA isolated from macronuclei of the ciliate, Tetrahymena pyriformis, has been found to contain [(6)N]methyl adenine (MeAde); this represents the first clear demonstration of significant amounts of MeAde in the DNA of a eucaryote. The amounts of macronuclear MeAde differed slightly between different strains of Tetrahymena, with approximately 0.65–0.80% of the adenine bases being methylated. The MeAde content of macronuclear DNA did not seem to vary in different physiological states. The level of MeAde in DNA isolated from micronuclei, on the other hand, was quite low (at least tenfold lower than in macronuclear DNA). The Rockefeller University Press 1973-03-01 /pmc/articles/PMC2108936/ /pubmed/4631666 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gorovsky, Martin A.
Hattman, Stanley
Pleger, Gloria Lorick
[(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS
title [(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS
title_full [(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS
title_fullStr [(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS
title_full_unstemmed [(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS
title_short [(6)N]METHYL ADENINE IN THE NUCLEAR DNA OF A EUCARYOTE, TETRAHYMENA PYRIFORMIS
title_sort [(6)n]methyl adenine in the nuclear dna of a eucaryote, tetrahymena pyriformis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108936/
https://www.ncbi.nlm.nih.gov/pubmed/4631666
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AT plegerglorialorick 6nmethyladenineinthenucleardnaofaeucaryotetetrahymenapyriformis

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