STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells

Normal and transformed baby hamster kidney (BHK) cells attach to Falcon polystyrene with the same first order rate constant. The longer the cells are attached to the bottles, the more difficult they are to remove. Sulfhydryl (—SH) binding reagents inhibit both the attachment of BHK cells and the inc...

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Detalles Bibliográficos
Autores principales: Grinnell, Frederick, Milam, Mary, Srere, Paul A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108941/
https://www.ncbi.nlm.nih.gov/pubmed/4734569
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author Grinnell, Frederick
Milam, Mary
Srere, Paul A.
author_facet Grinnell, Frederick
Milam, Mary
Srere, Paul A.
author_sort Grinnell, Frederick
collection PubMed
description Normal and transformed baby hamster kidney (BHK) cells attach to Falcon polystyrene with the same first order rate constant. The longer the cells are attached to the bottles, the more difficult they are to remove. Sulfhydryl (—SH) binding reagents inhibit both the attachment of BHK cells and the increase in adhesive strength of attached cells. Attached BHK cells bind fewer molecules of [1-(14)C]N-ethylamleimide (an —SH binding reagent) than do suspended cells. Incubation of cells with high concentrations of trypsin results in a reversible loss of cell adhesiveness. The recovery of adhesiveness of trypsin-treated cells is inhibited by cycloheximide.
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spelling pubmed-21089412008-05-01 STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells Grinnell, Frederick Milam, Mary Srere, Paul A. J Cell Biol Article Normal and transformed baby hamster kidney (BHK) cells attach to Falcon polystyrene with the same first order rate constant. The longer the cells are attached to the bottles, the more difficult they are to remove. Sulfhydryl (—SH) binding reagents inhibit both the attachment of BHK cells and the increase in adhesive strength of attached cells. Attached BHK cells bind fewer molecules of [1-(14)C]N-ethylamleimide (an —SH binding reagent) than do suspended cells. Incubation of cells with high concentrations of trypsin results in a reversible loss of cell adhesiveness. The recovery of adhesiveness of trypsin-treated cells is inhibited by cycloheximide. The Rockefeller University Press 1973-03-01 /pmc/articles/PMC2108941/ /pubmed/4734569 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Grinnell, Frederick
Milam, Mary
Srere, Paul A.
STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells
title STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells
title_full STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells
title_fullStr STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells
title_full_unstemmed STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells
title_short STUDIES ON CELL ADHESION : III. Adhesion of Baby Hamster Kidney Cells
title_sort studies on cell adhesion : iii. adhesion of baby hamster kidney cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108941/
https://www.ncbi.nlm.nih.gov/pubmed/4734569
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