THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS

Actinomycin D (AMD) at concentrations up to 0,25 µg/ml shows a differential effect on cell RNA synthesis and on the replication of an influenza virus in normal and virally transformed cells, both functions being more resistant to AMD in the transformed cell. A possible explanation for these differen...

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Detalles Bibliográficos
Autores principales: Williams, J. G., MacPherson, I. A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108966/
https://www.ncbi.nlm.nih.gov/pubmed/4734865
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author Williams, J. G.
MacPherson, I. A.
author_facet Williams, J. G.
MacPherson, I. A.
author_sort Williams, J. G.
collection PubMed
description Actinomycin D (AMD) at concentrations up to 0,25 µg/ml shows a differential effect on cell RNA synthesis and on the replication of an influenza virus in normal and virally transformed cells, both functions being more resistant to AMD in the transformed cell. A possible explanation for these differences in AMD sensitivity is provided by the observation that isotopically labeled AMD is maintained at a lower concentration in transformed BHK 21/13 (BHK) cells. There is evidence that the decreased sensitivity of the transformed cells to AMD is a result of maintenance of a lower internal concentration of the drug, since a correlation exists for a number of polyoma virus-transformed clones between sensitivity to and uptake of AMD.
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spelling pubmed-21089662008-05-01 THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS Williams, J. G. MacPherson, I. A. J Cell Biol Article Actinomycin D (AMD) at concentrations up to 0,25 µg/ml shows a differential effect on cell RNA synthesis and on the replication of an influenza virus in normal and virally transformed cells, both functions being more resistant to AMD in the transformed cell. A possible explanation for these differences in AMD sensitivity is provided by the observation that isotopically labeled AMD is maintained at a lower concentration in transformed BHK 21/13 (BHK) cells. There is evidence that the decreased sensitivity of the transformed cells to AMD is a result of maintenance of a lower internal concentration of the drug, since a correlation exists for a number of polyoma virus-transformed clones between sensitivity to and uptake of AMD. The Rockefeller University Press 1973-04-01 /pmc/articles/PMC2108966/ /pubmed/4734865 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Williams, J. G.
MacPherson, I. A.
THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS
title THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS
title_full THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS
title_fullStr THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS
title_full_unstemmed THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS
title_short THE DIFFERENTIAL EFFECT OF ACTINOMYCIN D IN NORMAL AND VIRUS-TRANSFORMED CELLS
title_sort differential effect of actinomycin d in normal and virus-transformed cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108966/
https://www.ncbi.nlm.nih.gov/pubmed/4734865
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