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A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS

A new method for the analysis of electron microscope autoradiographs is described which has two advantages over other methods. This method provides estimates for the activity in different regions of the section and takes into account the actual geometrical shape and position of different regions wit...

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Detalles Bibliográficos
Autores principales: Blackett, N. M., Parry, D. M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108967/
https://www.ncbi.nlm.nih.gov/pubmed/4691396
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author Blackett, N. M.
Parry, D. M.
author_facet Blackett, N. M.
Parry, D. M.
author_sort Blackett, N. M.
collection PubMed
description A new method for the analysis of electron microscope autoradiographs is described which has two advantages over other methods. This method provides estimates for the activity in different regions of the section and takes into account the actual geometrical shape and position of different regions within the section. Use is made of the measured distribution of grains about a thin line source to construct a hypothetical distribution of autoradiographic grains which is then compared with the actual distribution of grains. Different activities are then ascribed to various regions until a satisfactory agreement with the autoradiographic grains is obtained using the χ(2) significance. In the next paper, an example of the use of this method is given for the distribution of grains in the nucleus of erythroid bone marrow cells labeled with radioactive iron, (55)Fe.
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spelling pubmed-21089672008-05-01 A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS Blackett, N. M. Parry, D. M. J Cell Biol Article A new method for the analysis of electron microscope autoradiographs is described which has two advantages over other methods. This method provides estimates for the activity in different regions of the section and takes into account the actual geometrical shape and position of different regions within the section. Use is made of the measured distribution of grains about a thin line source to construct a hypothetical distribution of autoradiographic grains which is then compared with the actual distribution of grains. Different activities are then ascribed to various regions until a satisfactory agreement with the autoradiographic grains is obtained using the χ(2) significance. In the next paper, an example of the use of this method is given for the distribution of grains in the nucleus of erythroid bone marrow cells labeled with radioactive iron, (55)Fe. The Rockefeller University Press 1973-04-01 /pmc/articles/PMC2108967/ /pubmed/4691396 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Blackett, N. M.
Parry, D. M.
A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS
title A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS
title_full A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS
title_fullStr A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS
title_full_unstemmed A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS
title_short A NEW METHOD FOR ANALYZING ELECTRON MICROSCOPE AUTORADIOGRAPHS USING HYPOTHETICAL GRAIN DISTRIBUTIONS
title_sort new method for analyzing electron microscope autoradiographs using hypothetical grain distributions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108967/
https://www.ncbi.nlm.nih.gov/pubmed/4691396
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