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ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS
The effect of divalent cations on cell fusion by concentrated Sendai virus, inactivated by beta-propiolactone, was investigated using Vero and mouse L-929 cells in monolayers. With both cell lines, which are normally resistant to exogenous viral fusion, Cu(2+) in sublethal concentrations was found t...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1973
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108975/ https://www.ncbi.nlm.nih.gov/pubmed/4348787 |
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author | Wainberg, M. A. Howe, C. Godman, G. C. |
author_facet | Wainberg, M. A. Howe, C. Godman, G. C. |
author_sort | Wainberg, M. A. |
collection | PubMed |
description | The effect of divalent cations on cell fusion by concentrated Sendai virus, inactivated by beta-propiolactone, was investigated using Vero and mouse L-929 cells in monolayers. With both cell lines, which are normally resistant to exogenous viral fusion, Cu(2+) in sublethal concentrations was found to promote polykaryon formation to a marked degree. The simultaneous presence of Cu(2+) and virus was required for this effect, which was thought to be related to the cytotoxic action of Cu(2+) on the cell membrane. Accordingly, under standard conditions and in the absence of virus, leakage of isotopically labeled intracellular protein was shown to bear a quantitative relationship to Cu(2+) concentration. Concomitant changes in the membrane were seen electron microscopically to consist of loss of microvilli and the appearance of numerous vesicles on, or adjacent to, the membrane. The relationship of enhanced fusibility to these toxic changes was not further elucidated. The fusion-promoting effect of Cu(2+) far exceeded that of Ca(2+); and other cations tested had no effect. |
format | Text |
id | pubmed-2108975 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1973 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21089752008-05-01 ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS Wainberg, M. A. Howe, C. Godman, G. C. J Cell Biol Article The effect of divalent cations on cell fusion by concentrated Sendai virus, inactivated by beta-propiolactone, was investigated using Vero and mouse L-929 cells in monolayers. With both cell lines, which are normally resistant to exogenous viral fusion, Cu(2+) in sublethal concentrations was found to promote polykaryon formation to a marked degree. The simultaneous presence of Cu(2+) and virus was required for this effect, which was thought to be related to the cytotoxic action of Cu(2+) on the cell membrane. Accordingly, under standard conditions and in the absence of virus, leakage of isotopically labeled intracellular protein was shown to bear a quantitative relationship to Cu(2+) concentration. Concomitant changes in the membrane were seen electron microscopically to consist of loss of microvilli and the appearance of numerous vesicles on, or adjacent to, the membrane. The relationship of enhanced fusibility to these toxic changes was not further elucidated. The fusion-promoting effect of Cu(2+) far exceeded that of Ca(2+); and other cations tested had no effect. The Rockefeller University Press 1973-05-01 /pmc/articles/PMC2108975/ /pubmed/4348787 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Wainberg, M. A. Howe, C. Godman, G. C. ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS |
title | ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS |
title_full | ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS |
title_fullStr | ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS |
title_full_unstemmed | ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS |
title_short | ENHANCEMENT OF SENDAI VIRUS-MEDIATED CELL FUSION BY CUPRIC IONS |
title_sort | enhancement of sendai virus-mediated cell fusion by cupric ions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108975/ https://www.ncbi.nlm.nih.gov/pubmed/4348787 |
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