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ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION
A method for the ultrastructural localization of acyltransferase enzymes involved in phospholipid metabolism has been applied to the developing rat trigeminal nerve. Determination of acyltransferase levels in the nerve indicated that a peak of activity occurs at the 8th day after birth with gradual...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1973
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108996/ https://www.ncbi.nlm.nih.gov/pubmed/4349220 |
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author | Benes, Francine Higgins, Joan A. Barrnett, Russell J. |
author_facet | Benes, Francine Higgins, Joan A. Barrnett, Russell J. |
author_sort | Benes, Francine |
collection | PubMed |
description | A method for the ultrastructural localization of acyltransferase enzymes involved in phospholipid metabolism has been applied to the developing rat trigeminal nerve. Determination of acyltransferase levels in the nerve indicated that a peak of activity occurs at the 8th day after birth with gradual declines of activity up to 15 days. Morphological surveys and determinations of cholesterol levels suggested that heavy myelin formation occurs in the nerve during this latter period. Fixed nerves incubated in a medium for localization of acyltransferases indicated deposition of reaction product associated with Golgi cisternae, intracellular smooth vesicles, and the plasma membrane of the Schwann cell in the incipient stages of myelin formation. Golgi-derived vesicles appeared to move toward the Schwann cell surface and fuse with the plasma membrane. Activity continued to be detectable in the plasma membrane of the internal mesaxon as long as cytoplasm was evident and mature myelin membrane was not yet formed. Cells in which myelin formation appeared advanced showed little or no enzyme marker. Consistent with cytochemical observations were biochemical determinations of acyltransferases which showed high levels of the enzymes in microsomes, while no activity could be detected in the myelin fraction. Acyltransferase reaction product was also observed in the Golgi apparatus of ganglion cell bodies, axoplasmic smooth vesicles, and the axolemma. Localization of acyltransferase enzymes in Schwann cells, ganglion cell bodies, and axons during development of the nerve is discussed in relation to membrane biogenesis in the nervous system. |
format | Text |
id | pubmed-2108996 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1973 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21089962008-05-01 ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION Benes, Francine Higgins, Joan A. Barrnett, Russell J. J Cell Biol Article A method for the ultrastructural localization of acyltransferase enzymes involved in phospholipid metabolism has been applied to the developing rat trigeminal nerve. Determination of acyltransferase levels in the nerve indicated that a peak of activity occurs at the 8th day after birth with gradual declines of activity up to 15 days. Morphological surveys and determinations of cholesterol levels suggested that heavy myelin formation occurs in the nerve during this latter period. Fixed nerves incubated in a medium for localization of acyltransferases indicated deposition of reaction product associated with Golgi cisternae, intracellular smooth vesicles, and the plasma membrane of the Schwann cell in the incipient stages of myelin formation. Golgi-derived vesicles appeared to move toward the Schwann cell surface and fuse with the plasma membrane. Activity continued to be detectable in the plasma membrane of the internal mesaxon as long as cytoplasm was evident and mature myelin membrane was not yet formed. Cells in which myelin formation appeared advanced showed little or no enzyme marker. Consistent with cytochemical observations were biochemical determinations of acyltransferases which showed high levels of the enzymes in microsomes, while no activity could be detected in the myelin fraction. Acyltransferase reaction product was also observed in the Golgi apparatus of ganglion cell bodies, axoplasmic smooth vesicles, and the axolemma. Localization of acyltransferase enzymes in Schwann cells, ganglion cell bodies, and axons during development of the nerve is discussed in relation to membrane biogenesis in the nervous system. The Rockefeller University Press 1973-06-01 /pmc/articles/PMC2108996/ /pubmed/4349220 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Benes, Francine Higgins, Joan A. Barrnett, Russell J. ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION |
title | ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION |
title_full | ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION |
title_fullStr | ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION |
title_full_unstemmed | ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION |
title_short | ULTRASTRUCTURAL LOCALIZATION OF PHOSPHOLIPID SYNTHESIS IN THE RAT TRIGEMINAL NERVE DURING MYELINATION |
title_sort | ultrastructural localization of phospholipid synthesis in the rat trigeminal nerve during myelination |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2108996/ https://www.ncbi.nlm.nih.gov/pubmed/4349220 |
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