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QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin

Kinetic analysis of the initial ingestion rate of albumin-coated paraffin oil particles by human granulocytes and rabbit alveolar macrophages was undertaken to study the mechanism of action of cations and of heat-labile opsonin on engulfment. The rate of uptake of the particles was stimulated by Ca(...

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Autor principal: Stossel, Thomas P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109045/
https://www.ncbi.nlm.nih.gov/pubmed/4738105
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author Stossel, Thomas P.
author_facet Stossel, Thomas P.
author_sort Stossel, Thomas P.
collection PubMed
description Kinetic analysis of the initial ingestion rate of albumin-coated paraffin oil particles by human granulocytes and rabbit alveolar macrophages was undertaken to study the mechanism of action of cations and of heat-labile opsonin on engulfment. The rate of uptake of the particles was stimulated by Ca(++), Mg(++), Mn(++), or Co(++). At high concentrations (> 20 mM) Ca(++) and Mg(++) inhibited the rate of ingestion. Treatment of the particles with fresh serum (heat-labile opsonin) also stimulated the rate of ingestion. (125)I-labeled C3 was bound to the particles during opsonization. C3-deficient human serum lacked opsonic activity, which was restored by addition of purified C3. Normal, C2-deficient, and hereditary angioneurotic edema sera had equivalent opsonic activity. The serum opsonic activity thus involved C3 fixation to the particles by means of the properdin system. Although Mg(++) and heat-labile opsonin both accelerated the maximal rates of ingestion of the particles, neither altered the particle concentrations associated with one-half maximal ingestion rates. Opsonization of the particles markedly diminished the concentrations of divalent cations causing both stimulatory and inhibitory effects on ingestion rates and altered the shapes of the cation activation curves. (45)Ca was not bound to the particles during opsonization. The results are consistent with a mechanism whereby divalent cations and heat-labile opsonin activate ingestion by stimulating the work of engulfment rather than by merely enhancing cell-particle affinity, and whereby heat-labile opsonin acts by potentiating the effects of divalent cations.
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spelling pubmed-21090452008-05-01 QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin Stossel, Thomas P. J Cell Biol Article Kinetic analysis of the initial ingestion rate of albumin-coated paraffin oil particles by human granulocytes and rabbit alveolar macrophages was undertaken to study the mechanism of action of cations and of heat-labile opsonin on engulfment. The rate of uptake of the particles was stimulated by Ca(++), Mg(++), Mn(++), or Co(++). At high concentrations (> 20 mM) Ca(++) and Mg(++) inhibited the rate of ingestion. Treatment of the particles with fresh serum (heat-labile opsonin) also stimulated the rate of ingestion. (125)I-labeled C3 was bound to the particles during opsonization. C3-deficient human serum lacked opsonic activity, which was restored by addition of purified C3. Normal, C2-deficient, and hereditary angioneurotic edema sera had equivalent opsonic activity. The serum opsonic activity thus involved C3 fixation to the particles by means of the properdin system. Although Mg(++) and heat-labile opsonin both accelerated the maximal rates of ingestion of the particles, neither altered the particle concentrations associated with one-half maximal ingestion rates. Opsonization of the particles markedly diminished the concentrations of divalent cations causing both stimulatory and inhibitory effects on ingestion rates and altered the shapes of the cation activation curves. (45)Ca was not bound to the particles during opsonization. The results are consistent with a mechanism whereby divalent cations and heat-labile opsonin activate ingestion by stimulating the work of engulfment rather than by merely enhancing cell-particle affinity, and whereby heat-labile opsonin acts by potentiating the effects of divalent cations. The Rockefeller University Press 1973-08-01 /pmc/articles/PMC2109045/ /pubmed/4738105 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Stossel, Thomas P.
QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin
title QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin
title_full QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin
title_fullStr QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin
title_full_unstemmed QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin
title_short QUANTITATIVE STUDIES OF PHAGOCYTOSIS : Kinetic Effects of Cations and Heat-Labile Opsonin
title_sort quantitative studies of phagocytosis : kinetic effects of cations and heat-labile opsonin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109045/
https://www.ncbi.nlm.nih.gov/pubmed/4738105
work_keys_str_mv AT stosselthomasp quantitativestudiesofphagocytosiskineticeffectsofcationsandheatlabileopsonin