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CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM

The strength of attachment of normal and transformed baby hamster kidney cells was markedly increased when attached cells were treated with concanavalin A (Con A). The cells became less sensitive to detachment by physical shear or by treatment with trypsin or EDTA; however, their morphology, as obse...

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Detalles Bibliográficos
Autor principal: Grinnell, Frederick
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109071/
https://www.ncbi.nlm.nih.gov/pubmed/4201181
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author Grinnell, Frederick
author_facet Grinnell, Frederick
author_sort Grinnell, Frederick
collection PubMed
description The strength of attachment of normal and transformed baby hamster kidney cells was markedly increased when attached cells were treated with concanavalin A (Con A). The cells became less sensitive to detachment by physical shear or by treatment with trypsin or EDTA; however, their morphology, as observed by phase contrast microscopy, did not change. The effects of Con A were prevented by the simultaneous addition of either D-glucose or α-methyl-D-glucoside with the Con A. Also addition of these reagents to the attached cells after Con A treatment partially reversed the effects caused by Con A. Pre-treatment of the culture flasks with Con A before cell attachment resulted in an increase in the strength of cell attachment to the culture flasks as compared to untreated controls.
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spelling pubmed-21090712008-05-01 CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM Grinnell, Frederick J Cell Biol Article The strength of attachment of normal and transformed baby hamster kidney cells was markedly increased when attached cells were treated with concanavalin A (Con A). The cells became less sensitive to detachment by physical shear or by treatment with trypsin or EDTA; however, their morphology, as observed by phase contrast microscopy, did not change. The effects of Con A were prevented by the simultaneous addition of either D-glucose or α-methyl-D-glucoside with the Con A. Also addition of these reagents to the attached cells after Con A treatment partially reversed the effects caused by Con A. Pre-treatment of the culture flasks with Con A before cell attachment resulted in an increase in the strength of cell attachment to the culture flasks as compared to untreated controls. The Rockefeller University Press 1973-09-01 /pmc/articles/PMC2109071/ /pubmed/4201181 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Grinnell, Frederick
CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM
title CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM
title_full CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM
title_fullStr CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM
title_full_unstemmed CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM
title_short CONCANAVALIN A INCREASES THE STRENGTH OF BABY HAMSTER KIDNEY CELL ATTACHMENT TO SUBSTRATUM
title_sort concanavalin a increases the strength of baby hamster kidney cell attachment to substratum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109071/
https://www.ncbi.nlm.nih.gov/pubmed/4201181
work_keys_str_mv AT grinnellfrederick concanavalinaincreasesthestrengthofbabyhamsterkidneycellattachmenttosubstratum