DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES

With the use of the cytochemical stain for horseradish peroxidase of Graham and Karnovsky (1966. J. Histochem. Cytochem. 14:291), conjugates of horseradish peroxidase with ricin, wheat germ agglutinin, and phytohemagglutinin were employed for the morphologic demonstration of d-galactose (ricin), N-a...

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Autores principales: Gonatas, Nicholas K., Avrameas, Stratis
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109095/
https://www.ncbi.nlm.nih.gov/pubmed/4805008
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author Gonatas, Nicholas K.
Avrameas, Stratis
author_facet Gonatas, Nicholas K.
Avrameas, Stratis
author_sort Gonatas, Nicholas K.
collection PubMed
description With the use of the cytochemical stain for horseradish peroxidase of Graham and Karnovsky (1966. J. Histochem. Cytochem. 14:291), conjugates of horseradish peroxidase with ricin, wheat germ agglutinin, and phytohemagglutinin were employed for the morphologic demonstration of d-galactose (ricin), N-acetylglucosamine (wheat germ), and N-acetylgalactosamine (phytohemagglutinin) containing moieties on the surface of unfixed, or paraformaldehyde-fixed rat lymphoid cells. D-Galactose, or d-galactose containing disaccharides inhibited the interaction between ricin peroxidase and lymphoid cell surface; also, N-acetylglucosamine inhibited the wheat germ peroxidase-lymphoid cell interaction, but N-acetylgalactosamine failed to inhibit the reaction between phytohemagglutinin peroxidase and the surface of lymphoid cells.
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spelling pubmed-21090952008-05-01 DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES Gonatas, Nicholas K. Avrameas, Stratis J Cell Biol Article With the use of the cytochemical stain for horseradish peroxidase of Graham and Karnovsky (1966. J. Histochem. Cytochem. 14:291), conjugates of horseradish peroxidase with ricin, wheat germ agglutinin, and phytohemagglutinin were employed for the morphologic demonstration of d-galactose (ricin), N-acetylglucosamine (wheat germ), and N-acetylgalactosamine (phytohemagglutinin) containing moieties on the surface of unfixed, or paraformaldehyde-fixed rat lymphoid cells. D-Galactose, or d-galactose containing disaccharides inhibited the interaction between ricin peroxidase and lymphoid cell surface; also, N-acetylglucosamine inhibited the wheat germ peroxidase-lymphoid cell interaction, but N-acetylgalactosamine failed to inhibit the reaction between phytohemagglutinin peroxidase and the surface of lymphoid cells. The Rockefeller University Press 1973-11-01 /pmc/articles/PMC2109095/ /pubmed/4805008 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Gonatas, Nicholas K.
Avrameas, Stratis
DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES
title DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES
title_full DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES
title_fullStr DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES
title_full_unstemmed DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES
title_short DETECTION OF PLASMA MEMBRANE CARBOHYDRATES WITH LECTIN PEROXIDASE CONJUGATES
title_sort detection of plasma membrane carbohydrates with lectin peroxidase conjugates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109095/
https://www.ncbi.nlm.nih.gov/pubmed/4805008
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AT avrameasstratis detectionofplasmamembranecarbohydrateswithlectinperoxidaseconjugates

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