MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS

When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed: those in the heliozoan axoneme, the mitotic apparatus,...

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Detalles Bibliográficos
Autores principales: Tilney, Lewis G., Bryan, Joseph, Bush, Doris J., Fujiwara, Keigi, Mooseker, Mark S., Murphy, Douglas B., Snyder, Daniel H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1973
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109099/
https://www.ncbi.nlm.nih.gov/pubmed/4805001
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author Tilney, Lewis G.
Bryan, Joseph
Bush, Doris J.
Fujiwara, Keigi
Mooseker, Mark S.
Murphy, Douglas B.
Snyder, Daniel H.
author_facet Tilney, Lewis G.
Bryan, Joseph
Bush, Doris J.
Fujiwara, Keigi
Mooseker, Mark S.
Murphy, Douglas B.
Snyder, Daniel H.
author_sort Tilney, Lewis G.
collection PubMed
description When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed: those in the heliozoan axoneme, the mitotic apparatus, the contractile axostyle, repolymerized microtubules derived from the chick brain, the central pair in flagella, and the A tubules of flagella and the basal body. In all cases microtubules were composed of 13 equally spaced protofilaments. The B tubules in flagella and the basal body appear to be composed of 11 subunits. The connections of the B to the A and the C to the B are described. A model of a microtubule is presented.
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spelling pubmed-21090992008-05-01 MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS Tilney, Lewis G. Bryan, Joseph Bush, Doris J. Fujiwara, Keigi Mooseker, Mark S. Murphy, Douglas B. Snyder, Daniel H. J Cell Biol Article When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed: those in the heliozoan axoneme, the mitotic apparatus, the contractile axostyle, repolymerized microtubules derived from the chick brain, the central pair in flagella, and the A tubules of flagella and the basal body. In all cases microtubules were composed of 13 equally spaced protofilaments. The B tubules in flagella and the basal body appear to be composed of 11 subunits. The connections of the B to the A and the C to the B are described. A model of a microtubule is presented. The Rockefeller University Press 1973-11-01 /pmc/articles/PMC2109099/ /pubmed/4805001 Text en Copyright © 1973 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Tilney, Lewis G.
Bryan, Joseph
Bush, Doris J.
Fujiwara, Keigi
Mooseker, Mark S.
Murphy, Douglas B.
Snyder, Daniel H.
MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS
title MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS
title_full MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS
title_fullStr MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS
title_full_unstemmed MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS
title_short MICROTUBULES: EVIDENCE FOR 13 PROTOFILAMENTS
title_sort microtubules: evidence for 13 protofilaments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109099/
https://www.ncbi.nlm.nih.gov/pubmed/4805001
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