CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA

The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the...

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Detalles Bibliográficos
Autores principales: Allen, Howard J., Ault, Charles, Winzler, Richard J., Danielli, James F.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109129/
https://www.ncbi.nlm.nih.gov/pubmed/4588271
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author Allen, Howard J.
Ault, Charles
Winzler, Richard J.
Danielli, James F.
author_facet Allen, Howard J.
Ault, Charles
Winzler, Richard J.
Danielli, James F.
author_sort Allen, Howard J.
collection PubMed
description The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the isolated surface was quantitatively accounted for by the components analyzed. Chemical analyses showed the presence of lipid, protein, and carbohydrate. Mannose was the predominant neutral sugar. Analyses for three different strains of Amoeba were similar. Phosphate was found to be the major anionic group in the cell surface material. Sulfate, uronic acid, sialic acid, muramic acid, and nonamidated glutamic acid and aspartic acid were absent. Evidence is presented suggesting that the phosphate is associated with an unidentified nonreducing polyol.
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spelling pubmed-21091292008-05-01 CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA Allen, Howard J. Ault, Charles Winzler, Richard J. Danielli, James F. J Cell Biol Article The cell surface has been isolated from uninucleate, freshwater, phagocytic amoebae by a new procedure. Several criteria were employed to demonstrate purity of the cell surface fraction. All morphological components of the tripartite surface were present in the isolated surface and the weight of the isolated surface was quantitatively accounted for by the components analyzed. Chemical analyses showed the presence of lipid, protein, and carbohydrate. Mannose was the predominant neutral sugar. Analyses for three different strains of Amoeba were similar. Phosphate was found to be the major anionic group in the cell surface material. Sulfate, uronic acid, sialic acid, muramic acid, and nonamidated glutamic acid and aspartic acid were absent. Evidence is presented suggesting that the phosphate is associated with an unidentified nonreducing polyol. The Rockefeller University Press 1974-01-01 /pmc/articles/PMC2109129/ /pubmed/4588271 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Allen, Howard J.
Ault, Charles
Winzler, Richard J.
Danielli, James F.
CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA
title CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA
title_full CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA
title_fullStr CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA
title_full_unstemmed CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA
title_short CHEMICAL CHARACTERIZATION OF THE ISOLATED CELL SURFACE OF AMOEBA
title_sort chemical characterization of the isolated cell surface of amoeba
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109129/
https://www.ncbi.nlm.nih.gov/pubmed/4588271
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AT aultcharles chemicalcharacterizationoftheisolatedcellsurfaceofamoeba
AT winzlerrichardj chemicalcharacterizationoftheisolatedcellsurfaceofamoeba
AT daniellijamesf chemicalcharacterizationoftheisolatedcellsurfaceofamoeba

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