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LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA
Increasingly, physiological and biochemical data on the mammalian cornea have emphasized the importance of the endothelial rather than the epithelial layer in regulating the state of hydration of the cornea, via a transport ATPase. This view is supported by the observations reported here. With the E...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1974
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109228/ https://www.ncbi.nlm.nih.gov/pubmed/4274728 |
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author | Leuenberger, Peter M. Novikoff, Alex B. |
author_facet | Leuenberger, Peter M. Novikoff, Alex B. |
author_sort | Leuenberger, Peter M. |
collection | PubMed |
description | Increasingly, physiological and biochemical data on the mammalian cornea have emphasized the importance of the endothelial rather than the epithelial layer in regulating the state of hydration of the cornea, via a transport ATPase. This view is supported by the observations reported here. With the Ernst method, the first cytochemical procedure to be readily responsive to ions and ouabain, the major sites of enzyme reaction product are the intercellular spaces of the endothelial layer. This localization was not found by us with the so-called ATPase method of Wachstein and Meisel. |
format | Text |
id | pubmed-2109228 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1974 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21092282008-05-01 LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA Leuenberger, Peter M. Novikoff, Alex B. J Cell Biol Article Increasingly, physiological and biochemical data on the mammalian cornea have emphasized the importance of the endothelial rather than the epithelial layer in regulating the state of hydration of the cornea, via a transport ATPase. This view is supported by the observations reported here. With the Ernst method, the first cytochemical procedure to be readily responsive to ions and ouabain, the major sites of enzyme reaction product are the intercellular spaces of the endothelial layer. This localization was not found by us with the so-called ATPase method of Wachstein and Meisel. The Rockefeller University Press 1974-03-01 /pmc/articles/PMC2109228/ /pubmed/4274728 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Article Leuenberger, Peter M. Novikoff, Alex B. LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA |
title | LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA |
title_full | LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA |
title_fullStr | LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA |
title_full_unstemmed | LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA |
title_short | LOCALIZATION OF TRANSPORT ADENOSINE TRIPHOSPHATASE IN RAT CORNEA |
title_sort | localization of transport adenosine triphosphatase in rat cornea |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109228/ https://www.ncbi.nlm.nih.gov/pubmed/4274728 |
work_keys_str_mv | AT leuenbergerpeterm localizationoftransportadenosinetriphosphataseinratcornea AT novikoffalexb localizationoftransportadenosinetriphosphataseinratcornea |