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THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE

The distribution of the radioactivity from [(14)C]proline that is bound in cultured cells of Acer has been determined by electron microscope autoradiography. In this way proline may be related to the cell wall as a morphological entity rather than as a fraction in a biochemical separation of a heter...

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Autores principales: Steward, F. C., Israel, H. W., Salpeter, M. M.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109250/
https://www.ncbi.nlm.nih.gov/pubmed/4824292
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author Steward, F. C.
Israel, H. W.
Salpeter, M. M.
author_facet Steward, F. C.
Israel, H. W.
Salpeter, M. M.
author_sort Steward, F. C.
collection PubMed
description The distribution of the radioactivity from [(14)C]proline that is bound in cultured cells of Acer has been determined by electron microscope autoradiography. In this way proline may be related to the cell wall as a morphological entity rather than as a fraction in a biochemical separation of a heterogeneous crop of cells. The cells in culture may vary greatly. Some are active growing, turgid cells, with thin protoplasts tightly pressed against their walls; in others the protoplasts may spontaneously withdraw from the wall; in still others the protoplasts disorganize, and walls thicken and become sculptured as the cells differentiate and even senesce. Different culturing practices may affect the status of the cells, and this, in turn, affects the distribution of radioactivity from proline in the cells. Cells which are actively growing, turgid, and nucleated have the highest grain density in their protoplasts and nuclei; as the protoplasts of such cells withdraw from their walls, they retain the bulk of the radioactivity. On the other hand, in cells which have thickened walls and sparse protoplast contents, the radioactivity is accumulated in their walls. A high content of proline and hydroxyproline-rich protein is, therefore, not a necessary or invariable feature of the cell walls of cultured Acer cells but depends on the state of development of these cells.
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spelling pubmed-21092502008-05-01 THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE Steward, F. C. Israel, H. W. Salpeter, M. M. J Cell Biol Article The distribution of the radioactivity from [(14)C]proline that is bound in cultured cells of Acer has been determined by electron microscope autoradiography. In this way proline may be related to the cell wall as a morphological entity rather than as a fraction in a biochemical separation of a heterogeneous crop of cells. The cells in culture may vary greatly. Some are active growing, turgid cells, with thin protoplasts tightly pressed against their walls; in others the protoplasts may spontaneously withdraw from the wall; in still others the protoplasts disorganize, and walls thicken and become sculptured as the cells differentiate and even senesce. Different culturing practices may affect the status of the cells, and this, in turn, affects the distribution of radioactivity from proline in the cells. Cells which are actively growing, turgid, and nucleated have the highest grain density in their protoplasts and nuclei; as the protoplasts of such cells withdraw from their walls, they retain the bulk of the radioactivity. On the other hand, in cells which have thickened walls and sparse protoplast contents, the radioactivity is accumulated in their walls. A high content of proline and hydroxyproline-rich protein is, therefore, not a necessary or invariable feature of the cell walls of cultured Acer cells but depends on the state of development of these cells. The Rockefeller University Press 1974-03-01 /pmc/articles/PMC2109250/ /pubmed/4824292 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Steward, F. C.
Israel, H. W.
Salpeter, M. M.
THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE
title THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE
title_full THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE
title_fullStr THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE
title_full_unstemmed THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE
title_short THE LABELING OF CULTURED CELLS OF ACER WITH [(14)C]PROLINE AND ITS SIGNIFICANCE
title_sort labeling of cultured cells of acer with [(14)c]proline and its significance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109250/
https://www.ncbi.nlm.nih.gov/pubmed/4824292
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