ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis

Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for tissue processing. Electron-opaque deposits were found along the axonal plasma membranes, within mitochondria, and alon...

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Detalles Bibliográficos
Autores principales: Oschman, J. L., Hall, T. A., Peters, P. D., Wall, B. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109275/
https://www.ncbi.nlm.nih.gov/pubmed/4132064
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author Oschman, J. L.
Hall, T. A.
Peters, P. D.
Wall, B. J.
author_facet Oschman, J. L.
Hall, T. A.
Peters, P. D.
Wall, B. J.
author_sort Oschman, J. L.
collection PubMed
description Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for tissue processing. Electron-opaque deposits were found along the axonal plasma membranes, within mitochondria, and along the basal plasma membranes of Schwann cells. X-ray microprobe analysis (EMMA-4) yielded signals for calcium and phosphorus when deposits were probed, whereas these elements were not detected in the axoplasm.
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spelling pubmed-21092752008-05-01 ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis Oschman, J. L. Hall, T. A. Peters, P. D. Wall, B. J. J Cell Biol Article Giant axons from the squid, Loligo pealei, were fixed in glutaraldehyde and postfixed in osmium tetroxide. Calcium chloride (5 mM/liter) was added to all aqueous solutions used for tissue processing. Electron-opaque deposits were found along the axonal plasma membranes, within mitochondria, and along the basal plasma membranes of Schwann cells. X-ray microprobe analysis (EMMA-4) yielded signals for calcium and phosphorus when deposits were probed, whereas these elements were not detected in the axoplasm. The Rockefeller University Press 1974-04-01 /pmc/articles/PMC2109275/ /pubmed/4132064 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Oschman, J. L.
Hall, T. A.
Peters, P. D.
Wall, B. J.
ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis
title ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis
title_full ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis
title_fullStr ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis
title_full_unstemmed ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis
title_short ASSOCIATION OF CALCIUM WITH MEMBRANES OF SQUID GIANT AXON : Ultrastructure and Microprobe Analysis
title_sort association of calcium with membranes of squid giant axon : ultrastructure and microprobe analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109275/
https://www.ncbi.nlm.nih.gov/pubmed/4132064
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