Cargando…

EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE

The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated. Seven cloned PE cell lines were each grown in modi...

Descripción completa

Detalles Bibliográficos
Autores principales: Newsome, D. A., Fletcher, R. T., Robison, W. G., Kenyon, K. R., Chader, G. J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109292/
https://www.ncbi.nlm.nih.gov/pubmed/4363957
_version_ 1782139253834121216
author Newsome, D. A.
Fletcher, R. T.
Robison, W. G.
Kenyon, K. R.
Chader, G. J.
author_facet Newsome, D. A.
Fletcher, R. T.
Robison, W. G.
Kenyon, K. R.
Chader, G. J.
author_sort Newsome, D. A.
collection PubMed
description The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated. Seven cloned PE cell lines were each grown in modified Ham's F-12 medium alone (F-12), or in F-12 supplemented with either high molecular weight (H) or low molecular weight (L) fractions of chick embryo extract. Cells grown in F-12 alone or in L medium formed compact epithelial sheets, whereas cells grown in H had a fibrocytic appearance and formed poorly organized monolayers. In H plus BcAMP, cell morphology was more epithelioid than in H alone, and generally the monolayers appeared more differentiated. Under each of these three culture conditions, 2 x 10(-4) M BCAMP retarded the increase in cell number and decreased the final number of cells per culture dish, but had little effect on plating efficiency. BcAMP also increased the rate of cell adhesion to a plastic substratum. Pigmentation was marked in cultures grown in F-12 or in L alone, but the addition of BcAMP dramatically reduced visible pigmentation. This effect was reversed when BcAMP was removed from the culture medium. Thus BcAMP modifies cell and colonial morphology, rate of cell accumulation, adhesive properties, and pigmentation of normal PE cells.
format Text
id pubmed-2109292
institution National Center for Biotechnology Information
language English
publishDate 1974
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21092922008-05-01 EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE Newsome, D. A. Fletcher, R. T. Robison, W. G. Kenyon, K. R. Chader, G. J. J Cell Biol Article The effects of dibutyryl cyclic 3',5'-adenosine monophosphate (BcAMP) and Sephadex G-25 fractions of chick embryo extract on the growth rate, morphology, and pigmentation of normal chick retinal pigmented epithelium (PE) were investigated. Seven cloned PE cell lines were each grown in modified Ham's F-12 medium alone (F-12), or in F-12 supplemented with either high molecular weight (H) or low molecular weight (L) fractions of chick embryo extract. Cells grown in F-12 alone or in L medium formed compact epithelial sheets, whereas cells grown in H had a fibrocytic appearance and formed poorly organized monolayers. In H plus BcAMP, cell morphology was more epithelioid than in H alone, and generally the monolayers appeared more differentiated. Under each of these three culture conditions, 2 x 10(-4) M BCAMP retarded the increase in cell number and decreased the final number of cells per culture dish, but had little effect on plating efficiency. BcAMP also increased the rate of cell adhesion to a plastic substratum. Pigmentation was marked in cultures grown in F-12 or in L alone, but the addition of BcAMP dramatically reduced visible pigmentation. This effect was reversed when BcAMP was removed from the culture medium. Thus BcAMP modifies cell and colonial morphology, rate of cell accumulation, adhesive properties, and pigmentation of normal PE cells. The Rockefeller University Press 1974-05-01 /pmc/articles/PMC2109292/ /pubmed/4363957 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Newsome, D. A.
Fletcher, R. T.
Robison, W. G.
Kenyon, K. R.
Chader, G. J.
EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE
title EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE
title_full EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE
title_fullStr EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE
title_full_unstemmed EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE
title_short EFFECTS OF CYCLIC AMP AND SEPHADEX FRACTIONS OF CHICK EMBRYO EXTRACT ON CLONED RETINAL PIGMENTED EPITHELIUM IN TISSUE CULTURE
title_sort effects of cyclic amp and sephadex fractions of chick embryo extract on cloned retinal pigmented epithelium in tissue culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109292/
https://www.ncbi.nlm.nih.gov/pubmed/4363957
work_keys_str_mv AT newsomeda effectsofcyclicampandsephadexfractionsofchickembryoextractonclonedretinalpigmentedepitheliumintissueculture
AT fletcherrt effectsofcyclicampandsephadexfractionsofchickembryoextractonclonedretinalpigmentedepitheliumintissueculture
AT robisonwg effectsofcyclicampandsephadexfractionsofchickembryoextractonclonedretinalpigmentedepitheliumintissueculture
AT kenyonkr effectsofcyclicampandsephadexfractionsofchickembryoextractonclonedretinalpigmentedepitheliumintissueculture
AT chadergj effectsofcyclicampandsephadexfractionsofchickembryoextractonclonedretinalpigmentedepitheliumintissueculture