FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS

Sendai virus is able to induce the fusion of human erythrocytes. Bivalent cations or ATP are not essential for polyerythrocyte formation. High fusion indices were obtained when Sendai virus was added to cells incubated in the presence of both EDTA and iodoacetic acid. Human erythrocyte ghosts prepar...

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Detalles Bibliográficos
Autores principales: Peretz, Hava, Toister, Zivia, Laster, Yehudith, Loyter, Abraham
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1974
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109346/
https://www.ncbi.nlm.nih.gov/pubmed/4371393
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author Peretz, Hava
Toister, Zivia
Laster, Yehudith
Loyter, Abraham
author_facet Peretz, Hava
Toister, Zivia
Laster, Yehudith
Loyter, Abraham
author_sort Peretz, Hava
collection PubMed
description Sendai virus is able to induce the fusion of human erythrocytes. Bivalent cations or ATP are not essential for polyerythrocyte formation. High fusion indices were obtained when Sendai virus was added to cells incubated in the presence of both EDTA and iodoacetic acid. Human erythrocyte ghosts prepared by gradual hemolysis still retain the potential to undergo virus-induced fusion. Fusion of human red blood cells without the addition of viruses was obtained by incubation of erythrocytes at pH 10.5 in the presence of Ca(++) (40 mM) or by addition of phospholipase C Clostridium perfringens preparations to cells previously agglutinated or polylysine.
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spelling pubmed-21093462008-05-01 FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS Peretz, Hava Toister, Zivia Laster, Yehudith Loyter, Abraham J Cell Biol Article Sendai virus is able to induce the fusion of human erythrocytes. Bivalent cations or ATP are not essential for polyerythrocyte formation. High fusion indices were obtained when Sendai virus was added to cells incubated in the presence of both EDTA and iodoacetic acid. Human erythrocyte ghosts prepared by gradual hemolysis still retain the potential to undergo virus-induced fusion. Fusion of human red blood cells without the addition of viruses was obtained by incubation of erythrocytes at pH 10.5 in the presence of Ca(++) (40 mM) or by addition of phospholipase C Clostridium perfringens preparations to cells previously agglutinated or polylysine. The Rockefeller University Press 1974-10-01 /pmc/articles/PMC2109346/ /pubmed/4371393 Text en Copyright © 1974 by The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Peretz, Hava
Toister, Zivia
Laster, Yehudith
Loyter, Abraham
FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS
title FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS
title_full FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS
title_fullStr FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS
title_full_unstemmed FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS
title_short FUSION OF INTACT HUMAN ERYTHROCYTES AND ERYTHROCYTE GHOSTS
title_sort fusion of intact human erythrocytes and erythrocyte ghosts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109346/
https://www.ncbi.nlm.nih.gov/pubmed/4371393
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AT toisterzivia fusionofintacthumanerythrocytesanderythrocyteghosts
AT lasteryehudith fusionofintacthumanerythrocytesanderythrocyteghosts
AT loyterabraham fusionofintacthumanerythrocytesanderythrocyteghosts

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