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STUDIES ON DISPERSED PANCREATIC EXOCRINE CELLS : I. Dissociation Technique and Morphologic Characteristics of Separated Cells
A procedure for dissociation of the guinea pig pancreas into individual cells is described which employs enzymatic digestion with pure collagenase, chymotrypsin, and hyaluronidase, utilizes an interposed chelation of divalent cations by EDTA, and is terminated by gentle shearing. Yields of cells are...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1974
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109378/ https://www.ncbi.nlm.nih.gov/pubmed/4373477 |