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Two improved methods for preparing ferritin-protein conjugates for electron microscopy
Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferr...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1975
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109494/ https://www.ncbi.nlm.nih.gov/pubmed/803973 |
| _version_ | 1782139311324397568 |
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| collection | PubMed |
| description | Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin- antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells. |
| format | Text |
| id | pubmed-2109494 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1975 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21094942008-05-01 Two improved methods for preparing ferritin-protein conjugates for electron microscopy J Cell Biol Articles Two improved procedures were developed for activating ferritin so that the ferritin could be covalently linked to antibodies. One procedure involved use of a water-soluble carbodiimide and N-hydroxysuccinimide to prepare ferritin-containing activated esters. The other involved activation of the ferritin with excess glutaraldehyde. The ferritin- antibody conjugates prepared with the two procedures were shown to have a number of properties which made them suitable for locating antigenic components in cells. The Rockefeller University Press 1975-02-01 /pmc/articles/PMC2109494/ /pubmed/803973 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles Two improved methods for preparing ferritin-protein conjugates for electron microscopy |
| title | Two improved methods for preparing ferritin-protein conjugates for electron microscopy |
| title_full | Two improved methods for preparing ferritin-protein conjugates for electron microscopy |
| title_fullStr | Two improved methods for preparing ferritin-protein conjugates for electron microscopy |
| title_full_unstemmed | Two improved methods for preparing ferritin-protein conjugates for electron microscopy |
| title_short | Two improved methods for preparing ferritin-protein conjugates for electron microscopy |
| title_sort | two improved methods for preparing ferritin-protein conjugates for electron microscopy |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109494/ https://www.ncbi.nlm.nih.gov/pubmed/803973 |