Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining

A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells s...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1975
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109516/
https://www.ncbi.nlm.nih.gov/pubmed/49354
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collection PubMed
description A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps.
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spelling pubmed-21095162008-05-01 Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining J Cell Biol Articles A rapid method for the flow microfluorometric determination of the DNA content per cell is described. Incubation of cells in a hypotonic solution of propidium iodide results in disruption of the cell membrane and rapid staining of nuclear chromatin. DNA distribution histograms generated from cells stained by this method are identical to those generated after fixation and RNase digestion. In contrast to some earlier described methods, the present technique is rapid (5 min of processing), requires a minimal amount of material, and avoids formation of cell clumps. The Rockefeller University Press 1975-07-01 /pmc/articles/PMC2109516/ /pubmed/49354 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
title Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
title_full Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
title_fullStr Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
title_full_unstemmed Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
title_short Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
title_sort rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109516/
https://www.ncbi.nlm.nih.gov/pubmed/49354

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