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Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein
Pharmacological evidence is presented for the involvement of microtubules in the process of fast axoplasmic transport. A quantitative measure of the inhibition of axoplasmic transport in an in vitro preparation of rat sciatic nerve is described. The alkaloids colchicine, podophyllotoxin, and vinblas...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1975
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109605/ https://www.ncbi.nlm.nih.gov/pubmed/53233 |
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author | Paulson, J. C. McClure, W. O. |
author_facet | Paulson, J. C. McClure, W. O. |
author_sort | Paulson, J. C. |
collection | PubMed |
description | Pharmacological evidence is presented for the involvement of microtubules in the process of fast axoplasmic transport. A quantitative measure of the inhibition of axoplasmic transport in an in vitro preparation of rat sciatic nerve is described. The alkaloids colchicine, podophyllotoxin, and vinblastine, which are known both to disrupt microtubules and to bind to the protein subunit of microtubules, are inhibitors of axoplasmic transport. Lumicolchine and picropodophyllin, unlike their respective isomers colchicine and podophyllotoxin, are poor inhibitors of axoplasmic transport. The dissociation constants for the binding of colchicine, lumicolchicine, podophyllotoxin, and picropodophyllin to purified microtubule protein from rat brain have been measured. Inhibition of axoplasmic transport by these drugs correlates favorably with their affinities of microtubule protein. |
format | Text |
id | pubmed-2109605 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1975 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21096052008-05-01 Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein Paulson, J. C. McClure, W. O. J Cell Biol Journal Article Pharmacological evidence is presented for the involvement of microtubules in the process of fast axoplasmic transport. A quantitative measure of the inhibition of axoplasmic transport in an in vitro preparation of rat sciatic nerve is described. The alkaloids colchicine, podophyllotoxin, and vinblastine, which are known both to disrupt microtubules and to bind to the protein subunit of microtubules, are inhibitors of axoplasmic transport. Lumicolchine and picropodophyllin, unlike their respective isomers colchicine and podophyllotoxin, are poor inhibitors of axoplasmic transport. The dissociation constants for the binding of colchicine, lumicolchicine, podophyllotoxin, and picropodophyllin to purified microtubule protein from rat brain have been measured. Inhibition of axoplasmic transport by these drugs correlates favorably with their affinities of microtubule protein. The Rockefeller University Press 1975-11-01 /pmc/articles/PMC2109605/ /pubmed/53233 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Journal Article Paulson, J. C. McClure, W. O. Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein |
title | Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein |
title_full | Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein |
title_fullStr | Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein |
title_full_unstemmed | Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein |
title_short | Microtubules and axoplasmic transport. Inhibition of transport by podophyllotoxin: an interaction with microtubule protein |
title_sort | microtubules and axoplasmic transport. inhibition of transport by podophyllotoxin: an interaction with microtubule protein |
topic | Journal Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109605/ https://www.ncbi.nlm.nih.gov/pubmed/53233 |
work_keys_str_mv | AT paulsonjc microtubulesandaxoplasmictransportinhibitionoftransportbypodophyllotoxinaninteractionwithmicrotubuleprotein AT mcclurewo microtubulesandaxoplasmictransportinhibitionoftransportbypodophyllotoxinaninteractionwithmicrotubuleprotein |