Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes

In this communication, we have demonstrated that hydrolysis of the nucleotide sugar can cause errors in the detection of an ectoglycosyltransferase. Spleen cell suspensions can incorporate radioactivity when incubated with labeled UDP-galactose, but all the activity is due to decomposition of the nu...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109644/
https://www.ncbi.nlm.nih.gov/pubmed/1030711
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collection PubMed
description In this communication, we have demonstrated that hydrolysis of the nucleotide sugar can cause errors in the detection of an ectoglycosyltransferase. Spleen cell suspensions can incorporate radioactivity when incubated with labeled UDP-galactose, but all the activity is due to decomposition of the nucleotide sugar and uptake of the free sugar. The fibroblast cell lines can incroporate carbohydrate directly from UDP-galactose. Several criteria are presented with can be used to demonstrate that a nucleotide sugar is the direct carbohydrate donor.
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spelling pubmed-21096442008-05-01 Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes J Cell Biol Articles In this communication, we have demonstrated that hydrolysis of the nucleotide sugar can cause errors in the detection of an ectoglycosyltransferase. Spleen cell suspensions can incorporate radioactivity when incubated with labeled UDP-galactose, but all the activity is due to decomposition of the nucleotide sugar and uptake of the free sugar. The fibroblast cell lines can incroporate carbohydrate directly from UDP-galactose. Several criteria are presented with can be used to demonstrate that a nucleotide sugar is the direct carbohydrate donor. The Rockefeller University Press 1976-03-01 /pmc/articles/PMC2109644/ /pubmed/1030711 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes
title Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes
title_full Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes
title_fullStr Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes
title_full_unstemmed Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes
title_short Ectogalactosyltransferase studies in fibroblasts and concanavalin A- stimulated lymphocytes
title_sort ectogalactosyltransferase studies in fibroblasts and concanavalin a- stimulated lymphocytes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109644/
https://www.ncbi.nlm.nih.gov/pubmed/1030711

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