Evidence for RNA linked to nascent DNA in HeLa cells

Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled component revealed that its sedimentation coefficient is in the range of 4S and that it exists in a single- and double- stranded conformation. M...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109661/
https://www.ncbi.nlm.nih.gov/pubmed/1030704
_version_ 1782139362663727104
collection PubMed
description Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled component revealed that its sedimentation coefficient is in the range of 4S and that it exists in a single- and double- stranded conformation. Moreover, analysis by nitrocellulose chromatography and CsSO4 density sedimentation of the nascent DNA labeled with 3H-uridine revealed that it is covalently linked to short chains of RNA, indicating that in HeLa cells RNA primer is involved in DNA replication.
format Text
id pubmed-2109661
institution National Center for Biotechnology Information
language English
publishDate 1976
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21096612008-05-01 Evidence for RNA linked to nascent DNA in HeLa cells J Cell Biol Articles Rapidly labeled, i.e., nascent, DNA from HeLa cells was separated from the bulk DNA by ultracentrifugation. Further characterization of the rapidly labeled component revealed that its sedimentation coefficient is in the range of 4S and that it exists in a single- and double- stranded conformation. Moreover, analysis by nitrocellulose chromatography and CsSO4 density sedimentation of the nascent DNA labeled with 3H-uridine revealed that it is covalently linked to short chains of RNA, indicating that in HeLa cells RNA primer is involved in DNA replication. The Rockefeller University Press 1976-03-01 /pmc/articles/PMC2109661/ /pubmed/1030704 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Evidence for RNA linked to nascent DNA in HeLa cells
title Evidence for RNA linked to nascent DNA in HeLa cells
title_full Evidence for RNA linked to nascent DNA in HeLa cells
title_fullStr Evidence for RNA linked to nascent DNA in HeLa cells
title_full_unstemmed Evidence for RNA linked to nascent DNA in HeLa cells
title_short Evidence for RNA linked to nascent DNA in HeLa cells
title_sort evidence for rna linked to nascent dna in hela cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109661/
https://www.ncbi.nlm.nih.gov/pubmed/1030704

Ejemplares similares