Mating-reactive membrane vesicles from cilia of Paramecium caudatum

Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicle...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109699/
https://www.ncbi.nlm.nih.gov/pubmed/818093
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collection PubMed
description Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 nm. Electrophoretic analysis on SDS polyacrylamide gel revealed no significant difference in polypeptide patterns of the particles from the two complementary mating types.
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spelling pubmed-21096992008-05-01 Mating-reactive membrane vesicles from cilia of Paramecium caudatum J Cell Biol Articles Membrane vesicles with a high mating reactivity were obtained from cilia of Paramecium caudatum by treatment with a solution containing 2 M urea and 0.1 mM Na2-EDTA. All processes of conjugation were induced in cells of the complementary mating type by approximately 10 mug/ml proteins of the vesicles. Electron microscope observation showed that the membrane vesicles have a diameter of 100-150 nm. Electrophoretic analysis on SDS polyacrylamide gel revealed no significant difference in polypeptide patterns of the particles from the two complementary mating types. The Rockefeller University Press 1976-06-01 /pmc/articles/PMC2109699/ /pubmed/818093 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Mating-reactive membrane vesicles from cilia of Paramecium caudatum
title Mating-reactive membrane vesicles from cilia of Paramecium caudatum
title_full Mating-reactive membrane vesicles from cilia of Paramecium caudatum
title_fullStr Mating-reactive membrane vesicles from cilia of Paramecium caudatum
title_full_unstemmed Mating-reactive membrane vesicles from cilia of Paramecium caudatum
title_short Mating-reactive membrane vesicles from cilia of Paramecium caudatum
title_sort mating-reactive membrane vesicles from cilia of paramecium caudatum
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109699/
https://www.ncbi.nlm.nih.gov/pubmed/818093

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