DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment

We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phas...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109700/
https://www.ncbi.nlm.nih.gov/pubmed/1270517
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collection PubMed
description We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations.
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spelling pubmed-21097002008-05-01 DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment J Cell Biol Articles We have developed a method for permeabilizing CHO cells to nucleotides under conditions which allow most cells to remain viable. Permeabilized cells can carry out ATP-dependent, semiconservative synthesis of DNA. The data are consistent with the continuation of DNA synthesis in those cells in S phase at the time of treatment, possibly limited to completion of replicon synthesis without new initiations. The Rockefeller University Press 1976-06-01 /pmc/articles/PMC2109700/ /pubmed/1270517 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
title DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
title_full DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
title_fullStr DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
title_full_unstemmed DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
title_short DNA replication in Chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
title_sort dna replication in chinese hamster ovary cells made permeable to nucleotides by tween-80 treatment
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109700/
https://www.ncbi.nlm.nih.gov/pubmed/1270517

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