An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection

Purified alpha-bungarotoxin was isolated by chromatography and made radioactive with tritium ([3H]acetamidino-alpha-bungarotoxin). Infusions of [3H]alpha-bungarotoxin alone or preceded by tubocurarine or atropine were given into the third ventricle. 2. 12, or 24 h after injection the brains were pre...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109782/
https://www.ncbi.nlm.nih.gov/pubmed/1033189
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description Purified alpha-bungarotoxin was isolated by chromatography and made radioactive with tritium ([3H]acetamidino-alpha-bungarotoxin). Infusions of [3H]alpha-bungarotoxin alone or preceded by tubocurarine or atropine were given into the third ventricle. 2. 12, or 24 h after injection the brains were prepared for autoradiography. Injections of alpha-bungarotoxin (radioinert) in buffer, or of [3H]parathyroid hormone in buffer, served as controls. The various patterns of labeling suggest the presence of nicotinic-cholinergic neurons within the arcuate and basolateral regions of the hypothalamus including the supraoptic and suprachiasmatic nuclei and, in addition, the central nucleus of the amygdala.
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spelling pubmed-21097822008-05-01 An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection J Cell Biol Articles Purified alpha-bungarotoxin was isolated by chromatography and made radioactive with tritium ([3H]acetamidino-alpha-bungarotoxin). Infusions of [3H]alpha-bungarotoxin alone or preceded by tubocurarine or atropine were given into the third ventricle. 2. 12, or 24 h after injection the brains were prepared for autoradiography. Injections of alpha-bungarotoxin (radioinert) in buffer, or of [3H]parathyroid hormone in buffer, served as controls. The various patterns of labeling suggest the presence of nicotinic-cholinergic neurons within the arcuate and basolateral regions of the hypothalamus including the supraoptic and suprachiasmatic nuclei and, in addition, the central nucleus of the amygdala. The Rockefeller University Press 1976-12-01 /pmc/articles/PMC2109782/ /pubmed/1033189 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection
title An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection
title_full An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection
title_fullStr An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection
title_full_unstemmed An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection
title_short An autoradiographic analysis of [3H]alpha-bungarotoxin distribution in the rat brain after intraventricular injection
title_sort autoradiographic analysis of [3h]alpha-bungarotoxin distribution in the rat brain after intraventricular injection
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109782/
https://www.ncbi.nlm.nih.gov/pubmed/1033189

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