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Improved procedures for immunoferritin labeling of ultrathin frozen sections

In employing fixed frozen ultrathin sections as substrates for immunoferritin labeling of intracellular antigens, we have found that conventional glutaraldehyde fixation sometimes permits very little specific staining of the sections, either because it inactivates certain protein antigens, or becaus...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109786/
https://www.ncbi.nlm.nih.gov/pubmed/825524
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collection PubMed
description In employing fixed frozen ultrathin sections as substrates for immunoferritin labeling of intracellular antigens, we have found that conventional glutaraldehyde fixation sometimes permits very little specific staining of the sections, either because it inactivates certain protein antigens, or because it renders them inaccessible to the antibody stains. We have developed several fixation procedures that are chemically milder and allow a uniform but less extensive cross- linking of the specimen. With these procedures and precautions in the handling of the more fragile frozen sections, excellent structural preservation and specific immunoferritin labeling has been achieved with several systems.
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spelling pubmed-21097862008-05-01 Improved procedures for immunoferritin labeling of ultrathin frozen sections J Cell Biol Articles In employing fixed frozen ultrathin sections as substrates for immunoferritin labeling of intracellular antigens, we have found that conventional glutaraldehyde fixation sometimes permits very little specific staining of the sections, either because it inactivates certain protein antigens, or because it renders them inaccessible to the antibody stains. We have developed several fixation procedures that are chemically milder and allow a uniform but less extensive cross- linking of the specimen. With these procedures and precautions in the handling of the more fragile frozen sections, excellent structural preservation and specific immunoferritin labeling has been achieved with several systems. The Rockefeller University Press 1976-12-01 /pmc/articles/PMC2109786/ /pubmed/825524 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Improved procedures for immunoferritin labeling of ultrathin frozen sections
title Improved procedures for immunoferritin labeling of ultrathin frozen sections
title_full Improved procedures for immunoferritin labeling of ultrathin frozen sections
title_fullStr Improved procedures for immunoferritin labeling of ultrathin frozen sections
title_full_unstemmed Improved procedures for immunoferritin labeling of ultrathin frozen sections
title_short Improved procedures for immunoferritin labeling of ultrathin frozen sections
title_sort improved procedures for immunoferritin labeling of ultrathin frozen sections
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109786/
https://www.ncbi.nlm.nih.gov/pubmed/825524