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Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital

UDP-Glucuronyltransferase (GT) activity increases in chick embryo liver during culture from zero to a steady-state level at or above adult values. The GT activity (o-aminophenol as acceptor) is located entirely in the membranes of the endoplasmic reticulum (ER) and the question arises whether ER inc...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109821/
https://www.ncbi.nlm.nih.gov/pubmed/820699
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description UDP-Glucuronyltransferase (GT) activity increases in chick embryo liver during culture from zero to a steady-state level at or above adult values. The GT activity (o-aminophenol as acceptor) is located entirely in the membranes of the endoplasmic reticulum (ER) and the question arises whether ER increases along with GT. Earlier work showed that the synthesis and degradation rates of GT can be varied in culture over wide ranges by choosing embryo livers of different ages and both phenobarbital. In the present study we measured the GT activities and the concentrations of ER (using stereologic methods) in 5- and 11-day embryo liver during culture with and without phenobarbital. We found that GT and ER always increased in a constant ratio of 2.2 X 10(-9) U of GR activity per square micrometer of membrane, suggesting that the synthesis and degradation of GT are coupled to the synthesis and degradation of ER. A general structure for ER is proposed to explain this finding.
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spelling pubmed-21098212008-05-01 Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital J Cell Biol Articles UDP-Glucuronyltransferase (GT) activity increases in chick embryo liver during culture from zero to a steady-state level at or above adult values. The GT activity (o-aminophenol as acceptor) is located entirely in the membranes of the endoplasmic reticulum (ER) and the question arises whether ER increases along with GT. Earlier work showed that the synthesis and degradation rates of GT can be varied in culture over wide ranges by choosing embryo livers of different ages and both phenobarbital. In the present study we measured the GT activities and the concentrations of ER (using stereologic methods) in 5- and 11-day embryo liver during culture with and without phenobarbital. We found that GT and ER always increased in a constant ratio of 2.2 X 10(-9) U of GR activity per square micrometer of membrane, suggesting that the synthesis and degradation of GT are coupled to the synthesis and degradation of ER. A general structure for ER is proposed to explain this finding. The Rockefeller University Press 1976-08-01 /pmc/articles/PMC2109821/ /pubmed/820699 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital
title Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital
title_full Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital
title_fullStr Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital
title_full_unstemmed Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital
title_short Proliferation of endoplasmic reticulum with its enzyme, UDP- glucuronyltransferase, in chick embryo liver during culture. Effects of phenobarbital
title_sort proliferation of endoplasmic reticulum with its enzyme, udp- glucuronyltransferase, in chick embryo liver during culture. effects of phenobarbital
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109821/
https://www.ncbi.nlm.nih.gov/pubmed/820699