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Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process

The polycation polylysine, at different degrees of polymerization, was found to cause a marked inhibition of the conjugation process. Inhibition of conjugation by polylysine was highly dependent on the molecular weight of the polymer. When polylysine of a mol wt of 1,250 (degree of polymerization=6)...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109833/
https://www.ncbi.nlm.nih.gov/pubmed/820698
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collection PubMed
description The polycation polylysine, at different degrees of polymerization, was found to cause a marked inhibition of the conjugation process. Inhibition of conjugation by polylysine was highly dependent on the molecular weight of the polymer. When polylysine of a mol wt of 1,250 (degree of polymerization=6) was used, a concentration of 1.6 X 10(-5) M was required for a complete inhibition of conjugation, while only 2 X 10(-7) M of polylysine of a mol wt of 71,000 (degree of polymerization=340) was needed for the same effect. Polyaspartic acid prevented the inhibition of conjugation by polylysein. Chelators of bivalent metals such as O-phenanthroline (10(-3) M), EDTA (10(-3) M), and EGTA (5 X 10(-3) M) strongly inhibit the conjugation process in Tetrahymena pyriformis. The inhibition was partially prevented when bivalent metals such as Zn++, Fe++, and Ca++ were added together with the chelators. The lectin concanavalin A (25 mug/ml) completely prevented the conjugation process, while other lectins, such as phytohemagglutinin (500 mug/ml), soybean agglutinin (75 mug/ml) and wheat germ agglutinin (250 mug/ml) had no effect. Inhibition of conjugation by concanavalin A is completely reversible by 40 mM of alpha-methyl-D-mannoside.
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spelling pubmed-21098332008-05-01 Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process J Cell Biol Articles The polycation polylysine, at different degrees of polymerization, was found to cause a marked inhibition of the conjugation process. Inhibition of conjugation by polylysine was highly dependent on the molecular weight of the polymer. When polylysine of a mol wt of 1,250 (degree of polymerization=6) was used, a concentration of 1.6 X 10(-5) M was required for a complete inhibition of conjugation, while only 2 X 10(-7) M of polylysine of a mol wt of 71,000 (degree of polymerization=340) was needed for the same effect. Polyaspartic acid prevented the inhibition of conjugation by polylysein. Chelators of bivalent metals such as O-phenanthroline (10(-3) M), EDTA (10(-3) M), and EGTA (5 X 10(-3) M) strongly inhibit the conjugation process in Tetrahymena pyriformis. The inhibition was partially prevented when bivalent metals such as Zn++, Fe++, and Ca++ were added together with the chelators. The lectin concanavalin A (25 mug/ml) completely prevented the conjugation process, while other lectins, such as phytohemagglutinin (500 mug/ml), soybean agglutinin (75 mug/ml) and wheat germ agglutinin (250 mug/ml) had no effect. Inhibition of conjugation by concanavalin A is completely reversible by 40 mM of alpha-methyl-D-mannoside. The Rockefeller University Press 1976-08-01 /pmc/articles/PMC2109833/ /pubmed/820698 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process
title Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process
title_full Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process
title_fullStr Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process
title_full_unstemmed Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process
title_short Conjugation in Tetrahymena pyriformis. The effect of polylysine, concanavalin A, and bivalent metals on the conjugation process
title_sort conjugation in tetrahymena pyriformis. the effect of polylysine, concanavalin a, and bivalent metals on the conjugation process
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109833/
https://www.ncbi.nlm.nih.gov/pubmed/820698