Cargando…

Concentration of elements in mitotic chromatin as measured by x-ray microanalysis

Unfixed frozen-dried and uncoated tissue sections of the mouse duodenum were placed on carbon planchets and analyzed in a scanning electron microscope fitted with energy dispersive X-ray equipment. Computer analysis of the X-ray spectra allowed elemental microanalysis of the nucleus, cytoplasm, and...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109901/
https://www.ncbi.nlm.nih.gov/pubmed/856831
_version_ 1782139431053950976
collection PubMed
description Unfixed frozen-dried and uncoated tissue sections of the mouse duodenum were placed on carbon planchets and analyzed in a scanning electron microscope fitted with energy dispersive X-ray equipment. Computer analysis of the X-ray spectra allowed elemental microanalysis of the nucleus, cytoplasm, and mitotic chromatin regions in the cryptal and villus enterocytes. The peak to continuum ratio of S, Cl, K, and Ca were higher in mitotic chromatin than any of the other sites measured. The redistribution of Ca at mitosis is postulated to help explain both chromosome condensation and assembly of the mitotic spindle apparatus.
format Text
id pubmed-2109901
institution National Center for Biotechnology Information
language English
publishDate 1977
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21099012008-05-01 Concentration of elements in mitotic chromatin as measured by x-ray microanalysis J Cell Biol Articles Unfixed frozen-dried and uncoated tissue sections of the mouse duodenum were placed on carbon planchets and analyzed in a scanning electron microscope fitted with energy dispersive X-ray equipment. Computer analysis of the X-ray spectra allowed elemental microanalysis of the nucleus, cytoplasm, and mitotic chromatin regions in the cryptal and villus enterocytes. The peak to continuum ratio of S, Cl, K, and Ca were higher in mitotic chromatin than any of the other sites measured. The redistribution of Ca at mitosis is postulated to help explain both chromosome condensation and assembly of the mitotic spindle apparatus. The Rockefeller University Press 1977-04-01 /pmc/articles/PMC2109901/ /pubmed/856831 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Concentration of elements in mitotic chromatin as measured by x-ray microanalysis
title Concentration of elements in mitotic chromatin as measured by x-ray microanalysis
title_full Concentration of elements in mitotic chromatin as measured by x-ray microanalysis
title_fullStr Concentration of elements in mitotic chromatin as measured by x-ray microanalysis
title_full_unstemmed Concentration of elements in mitotic chromatin as measured by x-ray microanalysis
title_short Concentration of elements in mitotic chromatin as measured by x-ray microanalysis
title_sort concentration of elements in mitotic chromatin as measured by x-ray microanalysis
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109901/
https://www.ncbi.nlm.nih.gov/pubmed/856831