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Concentration of elements in mitotic chromatin as measured by x-ray microanalysis
Unfixed frozen-dried and uncoated tissue sections of the mouse duodenum were placed on carbon planchets and analyzed in a scanning electron microscope fitted with energy dispersive X-ray equipment. Computer analysis of the X-ray spectra allowed elemental microanalysis of the nucleus, cytoplasm, and...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1977
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109901/ https://www.ncbi.nlm.nih.gov/pubmed/856831 |
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collection | PubMed |
description | Unfixed frozen-dried and uncoated tissue sections of the mouse duodenum were placed on carbon planchets and analyzed in a scanning electron microscope fitted with energy dispersive X-ray equipment. Computer analysis of the X-ray spectra allowed elemental microanalysis of the nucleus, cytoplasm, and mitotic chromatin regions in the cryptal and villus enterocytes. The peak to continuum ratio of S, Cl, K, and Ca were higher in mitotic chromatin than any of the other sites measured. The redistribution of Ca at mitosis is postulated to help explain both chromosome condensation and assembly of the mitotic spindle apparatus. |
format | Text |
id | pubmed-2109901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1977 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21099012008-05-01 Concentration of elements in mitotic chromatin as measured by x-ray microanalysis J Cell Biol Articles Unfixed frozen-dried and uncoated tissue sections of the mouse duodenum were placed on carbon planchets and analyzed in a scanning electron microscope fitted with energy dispersive X-ray equipment. Computer analysis of the X-ray spectra allowed elemental microanalysis of the nucleus, cytoplasm, and mitotic chromatin regions in the cryptal and villus enterocytes. The peak to continuum ratio of S, Cl, K, and Ca were higher in mitotic chromatin than any of the other sites measured. The redistribution of Ca at mitosis is postulated to help explain both chromosome condensation and assembly of the mitotic spindle apparatus. The Rockefeller University Press 1977-04-01 /pmc/articles/PMC2109901/ /pubmed/856831 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Concentration of elements in mitotic chromatin as measured by x-ray microanalysis |
title | Concentration of elements in mitotic chromatin as measured by x-ray microanalysis |
title_full | Concentration of elements in mitotic chromatin as measured by x-ray microanalysis |
title_fullStr | Concentration of elements in mitotic chromatin as measured by x-ray microanalysis |
title_full_unstemmed | Concentration of elements in mitotic chromatin as measured by x-ray microanalysis |
title_short | Concentration of elements in mitotic chromatin as measured by x-ray microanalysis |
title_sort | concentration of elements in mitotic chromatin as measured by x-ray microanalysis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109901/ https://www.ncbi.nlm.nih.gov/pubmed/856831 |