Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster

DNA from purified mitochondria of Drosophila melanogaster can be isolated as supercoiled molecules which when nicked have a contour length of 5.9 micron. Partial denaturation mapping shows regional heterogeneity of base composition with one early denaturing region, with a calculated GC content close...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109910/
https://www.ncbi.nlm.nih.gov/pubmed/404300
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description DNA from purified mitochondria of Drosophila melanogaster can be isolated as supercoiled molecules which when nicked have a contour length of 5.9 micron. Partial denaturation mapping shows regional heterogeneity of base composition with one early denaturing region, with a calculated GC content close to zero, extending over 20% of the genome. DNA isolated from unfertilized eggs shows nuclear and mitochondrial DNA in equal proportions; we found no evidence of other cytoplasmic species.
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spelling pubmed-21099102008-05-01 Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster J Cell Biol Articles DNA from purified mitochondria of Drosophila melanogaster can be isolated as supercoiled molecules which when nicked have a contour length of 5.9 micron. Partial denaturation mapping shows regional heterogeneity of base composition with one early denaturing region, with a calculated GC content close to zero, extending over 20% of the genome. DNA isolated from unfertilized eggs shows nuclear and mitochondrial DNA in equal proportions; we found no evidence of other cytoplasmic species. The Rockefeller University Press 1977-05-01 /pmc/articles/PMC2109910/ /pubmed/404300 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster
title Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster
title_full Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster
title_fullStr Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster
title_full_unstemmed Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster
title_short Intramolecular heterogeneity of mitochondrial DNA of Drosophila melanogaster
title_sort intramolecular heterogeneity of mitochondrial dna of drosophila melanogaster
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109910/
https://www.ncbi.nlm.nih.gov/pubmed/404300

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