Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes

Mature ribosomes, their subunits, and their ribonucleoprotein precursors were extracted from HeLa cells and prepared for electron microscopy by a method based on adsorption of nucleic acid to a charged grid. Images so obtained revealed the presence of two types of ribonuclease-sensitive fibrils of c...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1977
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109921/
https://www.ncbi.nlm.nih.gov/pubmed/858742
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description Mature ribosomes, their subunits, and their ribonucleoprotein precursors were extracted from HeLa cells and prepared for electron microscopy by a method based on adsorption of nucleic acid to a charged grid. Images so obtained revealed the presence of two types of ribonuclease-sensitive fibrils of consistent morphology. One of these types of fibril is present on the 80S and 55S nucleolar ribonucleoprotein precursors and the mature 60S subunit. The other type is restricted to the 80S precursor. The morphology of the fibrils and their distribution in the various preparations suggest identification with known secondary structural features of isolated pre-rRNA.
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spelling pubmed-21099212008-05-01 Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes J Cell Biol Articles Mature ribosomes, their subunits, and their ribonucleoprotein precursors were extracted from HeLa cells and prepared for electron microscopy by a method based on adsorption of nucleic acid to a charged grid. Images so obtained revealed the presence of two types of ribonuclease-sensitive fibrils of consistent morphology. One of these types of fibril is present on the 80S and 55S nucleolar ribonucleoprotein precursors and the mature 60S subunit. The other type is restricted to the 80S precursor. The morphology of the fibrils and their distribution in the various preparations suggest identification with known secondary structural features of isolated pre-rRNA. The Rockefeller University Press 1977-05-01 /pmc/articles/PMC2109921/ /pubmed/858742 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes
title Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes
title_full Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes
title_fullStr Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes
title_full_unstemmed Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes
title_short Ribosome processing in HeLa cells. Studies on structural aspects of precursor and mature ribosomes
title_sort ribosome processing in hela cells. studies on structural aspects of precursor and mature ribosomes
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109921/
https://www.ncbi.nlm.nih.gov/pubmed/858742

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