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Newly synthesized acetylcholine receptors are located in the Golgi apparatus
Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]alpha-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated alpha-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface A Ch recept...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109962/ https://www.ncbi.nlm.nih.gov/pubmed/563402 |
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collection | PubMed |
description | Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]alpha-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated alpha-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface A Ch receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2 h. |
format | Text |
id | pubmed-2109962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1978 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21099622008-05-01 Newly synthesized acetylcholine receptors are located in the Golgi apparatus J Cell Biol Articles Chick skeletal muscle cells in tissue culture were fixed and treated with saponin to allow [125I]alpha-bungarotoxin access into the cells while preserving ultrastructure. The kinetics of binding of iodinated alpha-bungarotoxin to intracellular acetylcholine (ACh) receptors and to surface A Ch receptors were comparable. About half of the intracellular ACh receptors are newly synthesized and in the pathway leading to incorporation into the plasma membrane. Correlated electron microscope autoradiographic and kinetic studies of this receptor population suggest that a substantial fraction of the newly synthesized ACh receptors are located in the Golgi apparatus, where they reside for approx. 2 h. The Rockefeller University Press 1978-01-01 /pmc/articles/PMC2109962/ /pubmed/563402 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Newly synthesized acetylcholine receptors are located in the Golgi apparatus |
title | Newly synthesized acetylcholine receptors are located in the Golgi apparatus |
title_full | Newly synthesized acetylcholine receptors are located in the Golgi apparatus |
title_fullStr | Newly synthesized acetylcholine receptors are located in the Golgi apparatus |
title_full_unstemmed | Newly synthesized acetylcholine receptors are located in the Golgi apparatus |
title_short | Newly synthesized acetylcholine receptors are located in the Golgi apparatus |
title_sort | newly synthesized acetylcholine receptors are located in the golgi apparatus |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2109962/ https://www.ncbi.nlm.nih.gov/pubmed/563402 |