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Fluorescein colchicine. Synthesis, purification, and biological activity
The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chrom...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110006/ https://www.ncbi.nlm.nih.gov/pubmed/564913 |
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collection | PubMed |
description | The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of the kinetics of photodecomposition. FC inhibited [3H] colchicine binding to purified brain tubulin. The biological activity of FC was compared to the activity of unlabeled colchicine on mitosis, motility, secretion, and myogenesis. The effects of FC were identical to those of unlabeled colchicine in all biological systems tested. The results demonstrate that FC may be substituted for colchicine in biological experiments without significant loss in specificity or effectiveness. |
format | Text |
id | pubmed-2110006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1978 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21100062008-05-01 Fluorescein colchicine. Synthesis, purification, and biological activity J Cell Biol Articles The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of the kinetics of photodecomposition. FC inhibited [3H] colchicine binding to purified brain tubulin. The biological activity of FC was compared to the activity of unlabeled colchicine on mitosis, motility, secretion, and myogenesis. The effects of FC were identical to those of unlabeled colchicine in all biological systems tested. The results demonstrate that FC may be substituted for colchicine in biological experiments without significant loss in specificity or effectiveness. The Rockefeller University Press 1978-03-01 /pmc/articles/PMC2110006/ /pubmed/564913 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Fluorescein colchicine. Synthesis, purification, and biological activity |
title | Fluorescein colchicine. Synthesis, purification, and biological activity |
title_full | Fluorescein colchicine. Synthesis, purification, and biological activity |
title_fullStr | Fluorescein colchicine. Synthesis, purification, and biological activity |
title_full_unstemmed | Fluorescein colchicine. Synthesis, purification, and biological activity |
title_short | Fluorescein colchicine. Synthesis, purification, and biological activity |
title_sort | fluorescein colchicine. synthesis, purification, and biological activity |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110006/ https://www.ncbi.nlm.nih.gov/pubmed/564913 |