Cargando…

Fluorescein colchicine. Synthesis, purification, and biological activity

The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chrom...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1978
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110006/
https://www.ncbi.nlm.nih.gov/pubmed/564913
_version_ 1782139460642668544
collection PubMed
description The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of the kinetics of photodecomposition. FC inhibited [3H] colchicine binding to purified brain tubulin. The biological activity of FC was compared to the activity of unlabeled colchicine on mitosis, motility, secretion, and myogenesis. The effects of FC were identical to those of unlabeled colchicine in all biological systems tested. The results demonstrate that FC may be substituted for colchicine in biological experiments without significant loss in specificity or effectiveness.
format Text
id pubmed-2110006
institution National Center for Biotechnology Information
language English
publishDate 1978
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21100062008-05-01 Fluorescein colchicine. Synthesis, purification, and biological activity J Cell Biol Articles The synthesis of a fluorescent colchicine derivative permits the localization of colchicine-binding receptors in cells. Fluorescein colchicine (FC) was prepared by the addition of fluorescein isothiocyanate to deacetyl colchicine. The product, FC, was separated from the reactants by thin-layer chromatography (TLC). The purity of FC was demonstrated by TLC, UV spectral analysis, and analysis of the kinetics of photodecomposition. FC inhibited [3H] colchicine binding to purified brain tubulin. The biological activity of FC was compared to the activity of unlabeled colchicine on mitosis, motility, secretion, and myogenesis. The effects of FC were identical to those of unlabeled colchicine in all biological systems tested. The results demonstrate that FC may be substituted for colchicine in biological experiments without significant loss in specificity or effectiveness. The Rockefeller University Press 1978-03-01 /pmc/articles/PMC2110006/ /pubmed/564913 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Fluorescein colchicine. Synthesis, purification, and biological activity
title Fluorescein colchicine. Synthesis, purification, and biological activity
title_full Fluorescein colchicine. Synthesis, purification, and biological activity
title_fullStr Fluorescein colchicine. Synthesis, purification, and biological activity
title_full_unstemmed Fluorescein colchicine. Synthesis, purification, and biological activity
title_short Fluorescein colchicine. Synthesis, purification, and biological activity
title_sort fluorescein colchicine. synthesis, purification, and biological activity
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110006/
https://www.ncbi.nlm.nih.gov/pubmed/564913