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Cation-induced attachment of ciliary dynein cross-bridges

Isolated, demembranated Unio gill cilia that have been activated and fixed for thin-section electron microscopy in the presence of 2 mM MgSO4 have 87% of their outer dynein arms attached to an adjacent B subfiber. The distribution of attached arms is uniform with respect to doublet position in the c...

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Autor principal: Warner, F. D.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1978
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110146/
https://www.ncbi.nlm.nih.gov/pubmed/150425
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author Warner, F. D.
author_facet Warner, F. D.
author_sort Warner, F. D.
collection PubMed
description Isolated, demembranated Unio gill cilia that have been activated and fixed for thin-section electron microscopy in the presence of 2 mM MgSO4 have 87% of their outer dynein arms attached to an adjacent B subfiber. The distribution of attached arms is uniform with respect to doublet position in the cilium. When both 0.1 mM ATP and Mg++ are added to the activation and fixation solutions, the frequency of bridged arms is reduced to 48%. At the same time, the distribution of the attached arms appears to have been systematically modified with respect to doublet position and the active bend plane. Those doublet pairs positioned in the bend plane where interdoublet sliding is minimal retain a greater number of bridged arms than those doublet pairs positioned outside the bend plane where sliding is maximal. These observations imply a functional coupling of the Mg++-induced bridging of the dynein arms and the subsequent binding and hydrolysis of ATP that results in a force-generating cross-bridge cycle.
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spelling pubmed-21101462008-05-01 Cation-induced attachment of ciliary dynein cross-bridges Warner, F. D. J Cell Biol Rapid Communication Isolated, demembranated Unio gill cilia that have been activated and fixed for thin-section electron microscopy in the presence of 2 mM MgSO4 have 87% of their outer dynein arms attached to an adjacent B subfiber. The distribution of attached arms is uniform with respect to doublet position in the cilium. When both 0.1 mM ATP and Mg++ are added to the activation and fixation solutions, the frequency of bridged arms is reduced to 48%. At the same time, the distribution of the attached arms appears to have been systematically modified with respect to doublet position and the active bend plane. Those doublet pairs positioned in the bend plane where interdoublet sliding is minimal retain a greater number of bridged arms than those doublet pairs positioned outside the bend plane where sliding is maximal. These observations imply a functional coupling of the Mg++-induced bridging of the dynein arms and the subsequent binding and hydrolysis of ATP that results in a force-generating cross-bridge cycle. The Rockefeller University Press 1978-06-01 /pmc/articles/PMC2110146/ /pubmed/150425 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Rapid Communication
Warner, F. D.
Cation-induced attachment of ciliary dynein cross-bridges
title Cation-induced attachment of ciliary dynein cross-bridges
title_full Cation-induced attachment of ciliary dynein cross-bridges
title_fullStr Cation-induced attachment of ciliary dynein cross-bridges
title_full_unstemmed Cation-induced attachment of ciliary dynein cross-bridges
title_short Cation-induced attachment of ciliary dynein cross-bridges
title_sort cation-induced attachment of ciliary dynein cross-bridges
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110146/
https://www.ncbi.nlm.nih.gov/pubmed/150425
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