Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization

The preparation of a specific antiserum (anti-CaBP) against the calcium- binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence hist...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1978
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110157/
https://www.ncbi.nlm.nih.gov/pubmed/98531
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collection PubMed
description The preparation of a specific antiserum (anti-CaBP) against the calcium- binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding with the expression of the calcium transport function of the membrane. Furthermore, the CaBP is localized to the ectoderm of the CAM, appears to be exposed to the entire external surface of the ectoderm, and can be shown to be associated with cells enzymatically dissociated from the CAM. These results are consistent with a functional role of the CaBP in the CAM calcium transport process.
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spelling pubmed-21101572008-05-01 Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization J Cell Biol Articles The preparation of a specific antiserum (anti-CaBP) against the calcium- binding protein (CaBP) of the chorioallantoic membrane (CAM) is described. The anti-CaBP appeared to be specific for the CaBP by immunodiffusion and immunoelectrophoresis. Application of the anti-CaBP in immunofluorescence histochemistry revealed that the CaBP is present in the CAM only at developmental ages corresponding with the expression of the calcium transport function of the membrane. Furthermore, the CaBP is localized to the ectoderm of the CAM, appears to be exposed to the entire external surface of the ectoderm, and can be shown to be associated with cells enzymatically dissociated from the CAM. These results are consistent with a functional role of the CaBP in the CAM calcium transport process. The Rockefeller University Press 1978-06-01 /pmc/articles/PMC2110157/ /pubmed/98531 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
title Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
title_full Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
title_fullStr Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
title_full_unstemmed Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
title_short Calcium-binding protein of the chick chorioallantoic membrane. I. Immunohistochemical localization
title_sort calcium-binding protein of the chick chorioallantoic membrane. i. immunohistochemical localization
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110157/
https://www.ncbi.nlm.nih.gov/pubmed/98531

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