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A new method for excitation-contraction uncoupling in frog skeletal muscle
The mechanical activity of frog sartorius muscle fibers can be uncoupled from the electrical activity of their surface membranes by immersing the preparation in Ringer solution containing either 1.5 or 2.0 M of formamide for 15--20 min. This uncoupling is not reversed when the muscle is transferred...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1978
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110188/ https://www.ncbi.nlm.nih.gov/pubmed/568143 |
| _version_ | 1782139511572004864 |
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| collection | PubMed |
| description | The mechanical activity of frog sartorius muscle fibers can be uncoupled from the electrical activity of their surface membranes by immersing the preparation in Ringer solution containing either 1.5 or 2.0 M of formamide for 15--20 min. This uncoupling is not reversed when the muscle is transferred to normal frog Ringer solution. Formamide does not affect the electrical activity of the sciatic nerve branch, and both endplate potentials and miniature endplate potentials may be recorded from the uncoupled muscles. Prolonged exposure to formamide, beyond the time needed to paralyze, causes neuromuscular block. |
| format | Text |
| id | pubmed-2110188 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1978 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21101882008-05-01 A new method for excitation-contraction uncoupling in frog skeletal muscle J Cell Biol Articles The mechanical activity of frog sartorius muscle fibers can be uncoupled from the electrical activity of their surface membranes by immersing the preparation in Ringer solution containing either 1.5 or 2.0 M of formamide for 15--20 min. This uncoupling is not reversed when the muscle is transferred to normal frog Ringer solution. Formamide does not affect the electrical activity of the sciatic nerve branch, and both endplate potentials and miniature endplate potentials may be recorded from the uncoupled muscles. Prolonged exposure to formamide, beyond the time needed to paralyze, causes neuromuscular block. The Rockefeller University Press 1978-09-01 /pmc/articles/PMC2110188/ /pubmed/568143 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles A new method for excitation-contraction uncoupling in frog skeletal muscle |
| title | A new method for excitation-contraction uncoupling in frog skeletal muscle |
| title_full | A new method for excitation-contraction uncoupling in frog skeletal muscle |
| title_fullStr | A new method for excitation-contraction uncoupling in frog skeletal muscle |
| title_full_unstemmed | A new method for excitation-contraction uncoupling in frog skeletal muscle |
| title_short | A new method for excitation-contraction uncoupling in frog skeletal muscle |
| title_sort | new method for excitation-contraction uncoupling in frog skeletal muscle |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110188/ https://www.ncbi.nlm.nih.gov/pubmed/568143 |