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Alpha-actinin localization in the cleavage furrow during cytokinesis
We used antibodies against alpha-actinin and myosin labeled directly with contrasting fluorochromes to localize these contractile proteins simultaneously in dividing chick embryo cells. During mitosis anti- alpha-actinin stains diffusely the entire cytoplasm including the mitotic spindle, while in t...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1978
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110217/ https://www.ncbi.nlm.nih.gov/pubmed/359574 |
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collection | PubMed |
description | We used antibodies against alpha-actinin and myosin labeled directly with contrasting fluorochromes to localize these contractile proteins simultaneously in dividing chick embryo cells. During mitosis anti- alpha-actinin stains diffusely the entire cytoplasm including the mitotic spindle, while in the same cells intense antimyosin staining delineates the spindle. During cytokinesis both antibodies stain the cleavage furrow intensely, and until the midbody forms the two staining patterns in the same cell are identical at the resolution of the light microscope. Thereafter the anti-alpha-actinin staining of the furrow remains strong, but the antimyosin staining diminishes. These observations suggest that alpha-actinin participates along with actin and myosin in the membrane movements associated with cytokinesis. |
format | Text |
id | pubmed-2110217 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1978 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21102172008-05-01 Alpha-actinin localization in the cleavage furrow during cytokinesis J Cell Biol Articles We used antibodies against alpha-actinin and myosin labeled directly with contrasting fluorochromes to localize these contractile proteins simultaneously in dividing chick embryo cells. During mitosis anti- alpha-actinin stains diffusely the entire cytoplasm including the mitotic spindle, while in the same cells intense antimyosin staining delineates the spindle. During cytokinesis both antibodies stain the cleavage furrow intensely, and until the midbody forms the two staining patterns in the same cell are identical at the resolution of the light microscope. Thereafter the anti-alpha-actinin staining of the furrow remains strong, but the antimyosin staining diminishes. These observations suggest that alpha-actinin participates along with actin and myosin in the membrane movements associated with cytokinesis. The Rockefeller University Press 1978-10-01 /pmc/articles/PMC2110217/ /pubmed/359574 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Alpha-actinin localization in the cleavage furrow during cytokinesis |
title | Alpha-actinin localization in the cleavage furrow during cytokinesis |
title_full | Alpha-actinin localization in the cleavage furrow during cytokinesis |
title_fullStr | Alpha-actinin localization in the cleavage furrow during cytokinesis |
title_full_unstemmed | Alpha-actinin localization in the cleavage furrow during cytokinesis |
title_short | Alpha-actinin localization in the cleavage furrow during cytokinesis |
title_sort | alpha-actinin localization in the cleavage furrow during cytokinesis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110217/ https://www.ncbi.nlm.nih.gov/pubmed/359574 |