Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels

A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubu...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1978
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110225/
https://www.ncbi.nlm.nih.gov/pubmed/701376
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collection PubMed
description A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane.
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spelling pubmed-21102252008-05-01 Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels J Cell Biol Articles A procedure is described for localizing antigen-antibody complexes in sodium dodecyl sulfate (SDS) polyacrylamide gels using 125I-labeled protein A from Staphylococcus aureus. We use the procedure to probe antigenic cross-reactivities between Strongylocentrotus and Chlamydomonas alpha- and beta-tubulins; we also domonstrate how the procedure can detect minor antibody species in an antiserum directed against a cell membrane. The Rockefeller University Press 1978-10-01 /pmc/articles/PMC2110225/ /pubmed/701376 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels
title Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels
title_full Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels
title_fullStr Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels
title_full_unstemmed Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels
title_short Localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels
title_sort localization of cellular antigens in sodium dodecyl sulfate- polyacrylamide gels
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110225/
https://www.ncbi.nlm.nih.gov/pubmed/701376

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