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Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?

NADPH cytochrome c (cyt c) reductase and glucose-6-phosphatase, two enzymes thought to be restricted to the endoplasmic reticulum (ER) and widely used as ER markers, are present in isolated Golgi fractions assayed immediately after their isolation. Both enzymes are rapidly inactivated in fractions s...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1978
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110244/
https://www.ncbi.nlm.nih.gov/pubmed/214450
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description NADPH cytochrome c (cyt c) reductase and glucose-6-phosphatase, two enzymes thought to be restricted to the endoplasmic reticulum (ER) and widely used as ER markers, are present in isolated Golgi fractions assayed immediately after their isolation. Both enzymes are rapidly inactivated in fractions stored at 0 degrees C in 0.25 M sucrose, conditions which do not affect the activity of other enzymes in the same preparation. The inactivation process was shown to be dependent on time and protein concentration and could be prevented by EDTA and catalase. Morphological evidence shows that extensive membrane damage occurs parallel with the inactivation. Taken together with the immunological data in the companion paper, the findings indicate that the enzymes NADPH cyt c reductase and probably glucose-6-phosphate are indigenous components of Golgi membranes.
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spelling pubmed-21102442008-05-01 Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean? J Cell Biol Articles NADPH cytochrome c (cyt c) reductase and glucose-6-phosphatase, two enzymes thought to be restricted to the endoplasmic reticulum (ER) and widely used as ER markers, are present in isolated Golgi fractions assayed immediately after their isolation. Both enzymes are rapidly inactivated in fractions stored at 0 degrees C in 0.25 M sucrose, conditions which do not affect the activity of other enzymes in the same preparation. The inactivation process was shown to be dependent on time and protein concentration and could be prevented by EDTA and catalase. Morphological evidence shows that extensive membrane damage occurs parallel with the inactivation. Taken together with the immunological data in the companion paper, the findings indicate that the enzymes NADPH cyt c reductase and probably glucose-6-phosphate are indigenous components of Golgi membranes. The Rockefeller University Press 1978-11-01 /pmc/articles/PMC2110244/ /pubmed/214450 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?
title Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?
title_full Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?
title_fullStr Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?
title_full_unstemmed Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?
title_short Endoplasmic reticulum marker enzymes in Golgi fractions--what does this mean?
title_sort endoplasmic reticulum marker enzymes in golgi fractions--what does this mean?
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110244/
https://www.ncbi.nlm.nih.gov/pubmed/214450