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Distribution of newly formed ribosomal proteins in HeLa cell fractions
The distribution of newly formed ribosomal proteins between cytoplasmic, nucleoplasmic, and nucleolar fractions of HeLa cells was determined. All but a few of the newly formed ribosomal proteins were concentrated 10- to 50-fold in the nucleolus and two- to fivefold in the nucleoplasm. Nevertheless,...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1979
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110363/ https://www.ncbi.nlm.nih.gov/pubmed/457768 |
| _version_ | 1782139556706910208 |
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| collection | PubMed |
| description | The distribution of newly formed ribosomal proteins between cytoplasmic, nucleoplasmic, and nucleolar fractions of HeLa cells was determined. All but a few of the newly formed ribosomal proteins were concentrated 10- to 50-fold in the nucleolus and two- to fivefold in the nucleoplasm. Nevertheless, substantial amounts were found in the cytoplasm. Pretreatment of cells with actinomycin D to deplete the nucleolar pool of ribosomal precursor RNA had no effect on the concentration of newly formed ribosomal proteins in the nucleus, but did lead to an increased amount in the nucleoplasm at the expense of the nucleolus. |
| format | Text |
| id | pubmed-2110363 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 1979 |
| publisher | The Rockefeller University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-21103632008-05-01 Distribution of newly formed ribosomal proteins in HeLa cell fractions J Cell Biol Articles The distribution of newly formed ribosomal proteins between cytoplasmic, nucleoplasmic, and nucleolar fractions of HeLa cells was determined. All but a few of the newly formed ribosomal proteins were concentrated 10- to 50-fold in the nucleolus and two- to fivefold in the nucleoplasm. Nevertheless, substantial amounts were found in the cytoplasm. Pretreatment of cells with actinomycin D to deplete the nucleolar pool of ribosomal precursor RNA had no effect on the concentration of newly formed ribosomal proteins in the nucleus, but did lead to an increased amount in the nucleoplasm at the expense of the nucleolus. The Rockefeller University Press 1979-03-01 /pmc/articles/PMC2110363/ /pubmed/457768 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
| spellingShingle | Articles Distribution of newly formed ribosomal proteins in HeLa cell fractions |
| title | Distribution of newly formed ribosomal proteins in HeLa cell fractions |
| title_full | Distribution of newly formed ribosomal proteins in HeLa cell fractions |
| title_fullStr | Distribution of newly formed ribosomal proteins in HeLa cell fractions |
| title_full_unstemmed | Distribution of newly formed ribosomal proteins in HeLa cell fractions |
| title_short | Distribution of newly formed ribosomal proteins in HeLa cell fractions |
| title_sort | distribution of newly formed ribosomal proteins in hela cell fractions |
| topic | Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110363/ https://www.ncbi.nlm.nih.gov/pubmed/457768 |