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Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas
Frog exocrine pancreatic tissue was studied in vitro under conditions which maintain the differences between tissues from fasted and fed animals. Sodium dodecyl sulfate (SDS) gel electrophoresis after labeling with [14C]amino acids showed that feeding stimulated the synthesis of secretory proteins t...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1979
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110369/ https://www.ncbi.nlm.nih.gov/pubmed/313398 |
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collection | PubMed |
description | Frog exocrine pancreatic tissue was studied in vitro under conditions which maintain the differences between tissues from fasted and fed animals. Sodium dodecyl sulfate (SDS) gel electrophoresis after labeling with [14C]amino acids showed that feeding stimulated the synthesis of secretory proteins to the same relative degree as the overall protein synthesis. The intracellular transport of secretory proteins was studied by electronmicroscopy autoradiography after pulse- labeling with [3H]leucine. It was found that the transport route is similar under both feeding conditions. After their synthesis in the rough endoplasmic reticulum (RER), the proteins move through the peripheral elements and cisternae of the Golgi system into the condensing vacuoles. The velocity of the transport increases considerably after feeding. When frogs are fasted, the release of labeled proteins from the RER takes greater than 90 min, whereas after feeding, this happens within 30 min. Comparable differences were observed for transport through the Golgi system. The apparent differences between the frog and mammalian pancreas in the regulation of synthesis, intracellular transport, and secretion of proteins are discussed. |
format | Text |
id | pubmed-2110369 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1979 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21103692008-05-01 Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas J Cell Biol Articles Frog exocrine pancreatic tissue was studied in vitro under conditions which maintain the differences between tissues from fasted and fed animals. Sodium dodecyl sulfate (SDS) gel electrophoresis after labeling with [14C]amino acids showed that feeding stimulated the synthesis of secretory proteins to the same relative degree as the overall protein synthesis. The intracellular transport of secretory proteins was studied by electronmicroscopy autoradiography after pulse- labeling with [3H]leucine. It was found that the transport route is similar under both feeding conditions. After their synthesis in the rough endoplasmic reticulum (RER), the proteins move through the peripheral elements and cisternae of the Golgi system into the condensing vacuoles. The velocity of the transport increases considerably after feeding. When frogs are fasted, the release of labeled proteins from the RER takes greater than 90 min, whereas after feeding, this happens within 30 min. Comparable differences were observed for transport through the Golgi system. The apparent differences between the frog and mammalian pancreas in the regulation of synthesis, intracellular transport, and secretion of proteins are discussed. The Rockefeller University Press 1979-03-01 /pmc/articles/PMC2110369/ /pubmed/313398 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas |
title | Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas |
title_full | Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas |
title_fullStr | Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas |
title_full_unstemmed | Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas |
title_short | Effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas |
title_sort | effect of fasting and feeding on synthesis and intracellular transport of proteins in the frog exocrine pancreas |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2110369/ https://www.ncbi.nlm.nih.gov/pubmed/313398 |